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First published online April 18, 2008
Journal of Experimental Biology 211, 1448-1455 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.014951
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Role of the PGC-1 family in the metabolic adaptation of goldfish to diet and temperature

Christophe M. R. LeMoine, Christine E. Genge and Christopher D. Moyes*

Department of Biology, Queen's University, Kingston, ON K7L 3N6, Canada


Figure 1
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Fig. 1. Mitochondrial enzyme activities in red muscle (A,B), white muscle (C,D) and liver (E,F) of goldfish exposed to three acclimation temperatures (4, 20, 35°C) and three diets (fasted, control and high fat diet). Values are expressed as means + s.e.m., and are labelled with different letters to indicate statistical significance according to a Kruskall–Wallis test followed by a Mann–Whitney U-test, with N=5–7 for each treatment. CS, citrate synthase; COX, cytochrome oxidase.

 

Figure 2
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Fig. 2. Gene expression in red muscle (A), white muscle (B), heart (C) and liver (D) of goldfish exposed to three acclimation temperatures (4, 20, 35°C). Values are expressed as means + s.e.m. relative to controls (20°C and control diet fish). Different letters indicate statistically significant differences between treatments according to a Kruskall–Wallis test followed by a Mann–Whitney U-test (N=7–17). MCAD, medium chain acyl CoA dehydrogenase; NRF-1, nuclear respiratory factor-1; PPAR, peroxisome proliferator-activated receptor; PGC-1, PPAR{gamma} coactivator-1; OXPHOS, oxidative phosphorylation; other abbreviations as in Fig. 1.

 

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Fig. 3. Gene expression in red muscle (A), white muscle (B), heart (C) and liver (D) of goldfish exposed to three diets (fasted, control and high fat diet). Values are expressed as means + s.e.m. relative to controls (20°C and control diet fish). Different letters indicate statistically significant differences between treatments according to a Kruskall–Wallis test followed by a Mann–Whitney U-test (N=7–17). Abbreviations as in Fig. 2.

 

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Fig. 4. PGC-1β mRNA expressed as a proportion of PGC-1{alpha} + β transcript levels in red muscle (A), white muscle (B), heart (C) and liver (D) of goldfish exposed to three acclimation temperatures (4, 20, 35°C) and three dietary treatments (fasted, control and high fat diet). Bars represent means + s.e.m. Different letters indicate statistically significant differences between treatments according to a Kruskall–Wallis test followed by a Mann–Whitney U-test (N=7–17). Abbreviations as in Fig. 2.

 

Figure 5
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Fig. 5. Putative regulators of metabolic gene expression in goldfish tissue. Paired relative mRNA values for all tissues and all treatments were used; R2 values were determined by linear regression; all correlations were found to be significant (P<0.05; N=46–53 per tissue). Abbreviations as in Fig. 2.

 

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