First published online March 28, 2008
Journal of Experimental Biology 211, 1262-1269 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.013474
Cold induced changes of adenosine levels in common eelpout (Zoarces viviparus): a role in modulating cytochrome c oxidase expression
L. G. Eckerle,
M. Lucassen*,
T. Hirse and
H. O. Pörtner
Alfred Wegener Institute for Polar and Marine Research, Marine Animal
Physiology, Am Handelshafen 12, 27570 Bremerhaven, Germany
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Fig. 2. Cellular viability during primary culture of hepatocytes from common
eelpout (Z. viviparus). The number of viable cells (circles),
expressed relative to the initial cell number, and the fraction of dead cells
(triangles) in total cell counts were determined for cells from
warm-acclimated animals during primary culture at either 11°C (shaded
symbols) or 4°C (open symbols). Values are means ± s.e.m.
(N=3).
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Fig. 4. Relative expression of CS mRNA and of the different COX subunit mRNAs in
hepatocytes of cold- (white bars) and warm- (grey bars) acclimated eelpout
(Z. viviparus). Relative amounts of CS (A–C), COX2 (D–F)
and COX4 (G–I) mRNA were determined in freshly isolated hepatocytes
(A,D,G; N=4) and in cells after incubation at either 4 or 11°C
(B,C,E,F,H,I; N=5–8) under control conditions (white bars) or
with the addition of adenosine (hatched bars). mRNA levels in equal amounts of
total RNA were determined by RPA and normalized to the mean of freshly
isolated hepatocytes from warm-acclimated fish. *Significant
difference from control group incubated at the same temperature;
 significant difference from corresponding group incubated
at 4°C. Values are means ± s.e.m.
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Fig. 5. Influence of an adenosine receptor antagonist and agonist on adenosine
action. COX activities were determined in isolated hepatocytes after 48 or 72
h of incubation and normalized to the mean of the control group. Cells were
treated with the adenosine receptor antagonist 8-PT combined with adenosine
(ado) or with the adenosine agonist NECA instead of adenosine and are compared
to their analogous control and adenosine-treated groups. All reagents were
supplied at a concentration of 100 nmol ml–1. Cells were
prepared from warm-acclimated Z. viviparus and incubated at 11°C.
*Significant difference from control. Values are means ±
s.e.m. (N=4–6).
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© The Company of Biologists Ltd 2008
include samples with adenosine concentration below
detection limit. For these samples the detection limit of the method was used
for calculations. *Significant difference from other time points;
Significant difference
between acclimations; *significant difference from the control
group incubated at the same temperature;