First published online March 14, 2008
Journal of Experimental Biology 211, 1163-1168 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.015461
Identification of novel genes including Dermo-1, a marker of dermal differentiation, expressed in trout somitic external cells
Emmanuelle Dumont,
Cecile Rallière and
Pierre-Yves Rescan*
INRA (National Institute for Agricultural Research), Joint Research Unit
for Fish Physiology, Biodiversity and the Environment, INRA Scribe, IFR140,
Campus de Beaulieu, 35042, Rennes, France
View larger version (51K):
[in this window]
[in a new window]
|
Fig. 1. Expression of Pax7 in trout embryo. (A) Stage 16 embryo (approximately 40
somites). Frontal section through anterior tail: labelling is present in the
anterior domain of the somite (arrow indicates the boundary between two
somites). (B) Eyed-stage embryo. Transverse section through middle trunk:
labelling is present in the dorsal domain of the neural tube and at the
external surface of the somite. (C) Dual-colour in situ hybridisation
for Pax7 (dark blue) and slow myosin light chain 1 (MyLC1; red) transcripts.
Eyed-stage embryo. Transverse section through the middle trunk: Pax7-positive
somitic external cells (arrow) are lateral to the slow muscle fibres. n,
notochord; nt, neural tube; s, somite. Scale bar, 25 µm in A, 30 µm in B
and 35 µm in C.
|
|
View larger version (88K):
[in this window]
[in a new window]
|
Fig. 2. Expression of Dermo-1 in trout embryo. (A) Stage 10B embryo (approximately
10 somites). Lateral view: labelling is observed in the dorsal domain of the
somites (arrowheads) and in cephalic mesenchyme. (B) Stage 13 embryo
(approximately 30 somites). Lateral view: Dermo-1 transcript appears in the
ventral domain of rostral somites (arrowheads). (C) Stage 18 embryo
(segmentation is nearly complete). Lateral view: labelling has progressed up
to post-anal somites. (D,E) Stage 12 (20 somite) embryo. Transverse sections
through posterior (D) and anterior (E) trunk: labelling is initially present
in the dorsal domain of the somite and then appears in the ventral domain of
the somite (E: arrow). (F) Stage 40 somite embryo. Transverse section through
middle trunk: labelling narrows to the outermost domain of the somite and is
observed in sclerotome cells. (G) Stage 20 (somitogenesis is complete).
Transverse section through posterior trunk: Dermo-1 transcript is present in
somitic external cells (arrow), sclerotome cells and cells intercalated
between neural tube and myotome. (H–K) Dual-colour in situ
hybridisation for Dermo-1 (dark blue) and myogenin (red). (H–J) Serial
transverse sections through posterior (H), middle (I) and anterior trunk (J)
of a stage 12 embryo (approximately 20 somites). Dermo-1 and myogenin
expression progressively intermingle in the maturing somite. (K) Transverse
section through posterior trunk of an eyed-stage embryo. Dermo-1 labelling is
external to the myogenin-expressing primary myotome. n, notochord; nt, neural
tube; s, somite. Scale bar, 200 µm in A and B, 250 µm in C, 40 µm in
D, 30 µm in E, 35 µm in F, 45 µm in G and 35 µm in H–K.
|
|
View larger version (61K):
[in this window]
[in a new window]
|
Fig. 3. Expression of collectin subfamily member 12 in trout embryo. (A) Stage 10B
embryo (approximately 10 somites). Dorsal view (anterior to the top):
labelling is present in somites (arrow), lateral mesoderm and cephalic
mesenchyme. (B) Stage 12 embryo (approximately 25 somites). Lateral view:
labelling progresses caudally as somites form. (C) Eyed-stage embryo. Lateral
view: labelling has progressed up to the most caudal somites. (D) Stage 10B
embryo. Transverse section through the middle trunk: labelling is confined to
a narrow dorsal domain of the somite (arrow). (E) Stage 20 somite. Transverse
section through the anterior trunk: labelling extends to a large domain of the
somite and is detectable in sclerotome cells (arrow). (F) Stage 35 somite
embryo. Transverse section through the anterior trunk: labelling has narrowed
to the external cells and is present in sclerotome cells surrounding the
notochord. (G) Stage 20 embryo (segmentation is complete). Transverse section
through the middle trunk: labelling is evident in the external cells (arrow),
the facing epidermis (arrowhead) and in sclerotome cells. Scale bar, 150 µm
in A, 200 µm in B, 300 µm in C, 20 µm in D, 40 µm in E and F, and
50 µm in G.
|
|
View larger version (72K):
[in this window]
[in a new window]
|
Fig. 4. Expression of Seraf in trout embryo. (A) Stage 10B embryo (approximately 10
somites). Dorsal view (anterior to the top): labelling is observed in somites
(arrowheads) as well as in differentiating notochord (arrow). (B) Stage 20
embryo (segmentation is complete). Lateral view: labelling is observed in
somites and cephalic mesenchyme. (C,D) Stage 13 embryo (approximately 30
somites). Transverse sections through posterior (C) and anterior trunk (D)
showing the progressive disappearance of Seraf transcript in the notochord and
the medial part of the somite. (E) Eyed-stage embryo. Transverse section
through the middle trunk. Seraf transcript is observed in the ventral and
dorsal tips of the myotome (arrowheads), in external cells (arrows) and at the
dorsal midline. Scale bar, 100 µm in A, 200 µm in B, 25 µm in C, and
30 µm in D and E.
|
|
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
© The Company of Biologists Ltd 2008
