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First published online March 14, 2008
Journal of Experimental Biology 211, 1057-1062 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.013433
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Thermodynamics of oxygenation-linked proton and lactate binding govern the temperature sensitivity of O2 binding in crustacean (Carcinus maenas) hemocyanin

Roy E. Weber*, Jane W. Behrens{dagger}, Hans Malte and Angela Fago

Zoophysiology, Department of Biological Sciences, University of Aarhus, CF Møllers Alle 1131, DK 8000 Aarhus, Denmark


Figure 1
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  		Fig. 1. (A) Effects of increasing lactate concentrations on hemolymph P50 values at pH 7.00 and 7.70 and 20°C. Hc subunit concentration, 0.85 mmol l–1.

 

Figure 2
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  		Fig. 2. Extended Hill plots of C. maenas Hc measured at 10°C in the absence (circles) and presence of 10 mmol l–1 (triangles) and 50 mmol l–1 (squares) L-lactate. Hc subunit concentration, 2.76 mmol l–1. Open and closed symbols are data points where the MWC equation was fitted with q floating and fixed at 4, respectively, during the iteration (see Eqn 1). Mean pH=7.843±0.009.

 

Figure 3
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  		Fig. 3. P50 and n50 values of C. maenas Hc measured at 10°C (circles) and 20°C (triangles) in the absence of added lactate (open symbols) and presence (closed symbols) of 10 mmol l–1 L-lactate. Hc subunit concentration, 1.11 mmol l–1.

 

Figure 4
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  		Fig. 4. O2 equilibrium curves of C. maenas Hc at 10 and 20°C and pH 7.8 and 7.0, interpolated from data in Fig. 3.

 

Figure 5
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  		Fig. 5. (Top) Overall oxygenation enthalpies ({Delta}H') derived using the van't Hoff isochore and P50 values at 10 and 20°C and different pH values (Fig. 3), in the absence of added lactate (A) and the presence of 10 mmol l–1 lactate (B). (Bottom) The interpolated, pH-dependent difference between these values (B–A).

 

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© The Company of Biologists Ltd 2008