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First published online February 1, 2008
Journal of Experimental Biology 211, 539-547 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.009175
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Sodium uptake in different life stages of crustaceans: the water flea Daphnia magna Strauss

Adalto Bianchini1,* and Chris M. Wood2

1 Fundação Universidade Federal do Rio Grande, Departamento de Ciências Fisiológicas, Campus Carreiros, Av. Itália s/n, 96.201-900 Rio Grande, RS, Brazil
2 McMaster University, Department of Biology, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada


Figure 1
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  		Fig. 1. Influence of wet body mass (ww) on mass-specific Na+ uptake rate (influx) in the water flea Daphnia magna. Note that both axes are logarithmic.

 

Figure 2
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  		Fig. 2. Kinetics of mass-specific Na+ uptake rate in neonate and adult water flea Daphnia magna as a function of sodium concentration. Data are expressed as mean ± 1 s.e.m. (N=6). Kinetic parameters (Michaelis constant [Km] and maximal velocity [Vmax]) for sodium uptake in neonate and adult daphnids were determined by means of nonlinear regression analyses (one-site binding), as previously described (Bianchini and Wood, 2003Go).

 

Figure 3
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  		Fig. 3. Whole-body Na+ uptake rate in neonate and adult water flea Daphnia magna after pre-exposure to 1% dimethylsulfoxide (DMSO), in the absence of Cl– (replaced with equimolar gluconate) in the external medium (Gluco) or after pre-exposure to 2,4,6-triaminopyrimidine (TAP, 10–3 mol l–1). Data are expressed as mean ± 1 s.e.m. (N=6). Note the different axis scale for neonate (left) and adult (right) daphnids.

 

Figure 4
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  		Fig. 4. Whole-body Na+ uptake rate in neonate and adult water flea Daphnia magna after pre-exposure to amiloride (Amilo, 10–3 mol l–1), phenamil (Phena, 10–4 mol l–1) or bafilomycin A1 (Bafilo, 5x10–7 mol l–1). Data are expressed as mean ± 1 s.e.m. (N=6). Note the different axis scale for neonate (left) and adult (right) daphnids.

 

Figure 5
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  		Fig. 5. Whole-body Na+ uptake rate in neonate and adult water flea Daphnia magna after pre-exposure to furosemide (furo, 10–3 mol l–1), bumetanide (bume, 10–3 mol l–1) or thiazide (thiaz, 10–3 mol l–1). Data are expressed as mean ± 1 s.e.m. (N=6). Note the different axis scale for neonate (left) and adult (right) daphnids.

 

Figure 6
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  		Fig. 6. Whole-body Na+ uptake rate in neonate and adult water flea Daphnia magna after pre-exposure to 4,4' diisothiocyanostilbene-2,2' disulfonic acid (DIDS, 10–3 mol l–1), diphenylamine-2-carboxylate (DPC, 10–3 mol l–1) or acetazolamide (Aceta, 10–3 mol l–1). Data are expressed as mean ± 1 s.e.m. (N=6). Note the different axis scale for neonate (left) and adult (right) daphnids.

 

Figure 7
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  		Fig. 7. Functional models of ion transport mechanisms involved in whole-body Na+ uptake in neonate (A) and adult (B) water flea Daphnia magna. CA, carbonic anhydrase. Solid and broken lines indicate transport and diffusion, respectively. ? indicates the need for more data to confirm or not the presence of the mechanism of transport.

 

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© The Company of Biologists Ltd 2008