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First published online October 31, 2008
Journal of Experimental Biology 211, 3544-3553 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.020644

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Mechanism and rate of glucose absorption differ between an Australian honeyeater (Meliphagidae) and a lorikeet (Loriidae)

Kathryn R. Napier^*, Todd J. McWhorter and Patricia A. Fleming

School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia

View larger version (36K): [in this window] [in a new window]   Fig. 1. Plots of the mean dose-corrected plasma concentrations of D-[14C]glucose, [14C]3-OMG and L-[3H]glucose as a function of time since (A–C) injection (insets: semi-loge plots) and (D–F) oral gavage of the probes. In (A,D) rainbow lorikeets N=8 for all time points except at 20 min after injection, where N=6 and (B,C,E,F) red wattlebirds N=8 for all time points except at 30 min after injection of D-[14C]glucose, N=7. The units for concentration are proportion per µg of plasma, as values of d.p.m. µg–1 plasma were normalised to injected or oral dose for each individual. Filled circles, L-[3H]glucose; unfilled circles, D-[14C]glucose; and unfilled inverted triangles, [14C]3-OMG.

View larger version (27K): [in this window] [in a new window]   Fig. 2. Plots of (A–C) mean apparent rates of absorption (fraction of the oral dose absorbed per minute) of D-[3H]glucose, [14C]3-OMG and L-[3H]glucose, and (D–F) the mean ratio of L-glucose to D-glucose or 3-OMG cumulative fractional absorption (fraction of the amount of the oral dose ultimately absorbed by each time point). In (A,D) rainbow lorikeets and (B,C,E,F) red wattlebirds N=8 for all time points for both species. Measurements were made under conditions saturating for mediated glucose transporters in both species. Filled circles in A–C, L-[3H]glucose; unfilled circles, D-[14C]glucose; unfilled inverted triangles, [14C]3-OMG. Asterisks in B and C indicate a significant difference in apparent absorption rate of the two probes when data for individual birds were compared for each sampling time point separately (RM-ANOVA, P<0.05). Note different y-axis scales for A–C.

View larger version (28K): [in this window] [in a new window]   Fig. 3. Apparent total percentage of the dose of L-[3H]glucose and 14C (administered as D-[14C]glucose) recovered in (A) intestinal contents, (B) intestinal tissues (including all section from the proventriculus distally), (C) plasma, (D) liver, (E) all other tissues including kidney, pectoralis and brain, and (F) summed for intestinal contents and all tissues measured in rainbow lorikeets (left hand panels) and red wattlebirds (right hand panels) at varying times of euthanasia (N=1 per species at each time point). Total recovery for each tissue was estimated by extrapolating the concentration of radioactivity in sub-samples to the whole tissue mass, and is expressed as a percentage of the dose delivered by oral gavage.

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