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First published online September 19, 2008
Journal of Experimental Biology 211, 3160-3166 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.010124

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Natural variation in plasticity of glucose homeostasis and food intake

Karla R. Kaun, Munmun Chakaborty-Chatterjee and Marla B. Sokolowski^*

Department of Biology, University of Toronto, 3359 Mississauga Road, Mississauga, Ontario, Canada L5L 1C6

View larger version (21K): [in this window] [in a new window]   Fig. 1 Natural variation in nutrient storage due to for. (A) Rover (forR) larvae store a larger proportion of ingested 14C in their lipid stores whereas sitter larvae (fors and fors2) store a larger proportion in their carbohydrate stores. (B) Well-fed mid-third-instar forR, fors and fors2 larvae differ in total body carbohydrate but not lipid or protein stores. (C) Well-fed forR larvae have significantly lower carbohydrate levels in fat body but not in hemolymph (D) compared with fors and fors2 larvae. Error bars indicate means ± s.e.m.

View larger version (19K): [in this window] [in a new window]   Fig. 2 Natural variation in nutrient store expenditure following acute food deprivation. (A) Acute food deprivation does not affect total protein levels in rovers (forR) or sitters (fors and fors2). (B) Three hours of acute food deprivation significantly decreases total body lipid levels in rover but not in sitter larvae. (C) Acute food deprivation does not affect whole body carbohydrate levels despite rover larvae showing significantly lower total body carbohydrate levels compared with sitter larvae. (D) Acute food deprivation decreases hemolymph carbohydrate levels in forR larvae but not fors or fors2 larvae. (E) Acute food deprivation does not affect fat body carbohydrate levels; forR larvae have significantly less carbohydrate in their fat bodies than fors or fors2 larvae. Error bars indicate means ± s.e.m.

View larger version (19K): [in this window] [in a new window]   Fig. 3 for affects recovery from acute food deprivation. (A) After one hour of food deprivation, sitter (fors and fors2) larvae increase their food intake to a greater extent than rover (forR) larvae. After two and three hours of food deprivation, there were no significant differences in food intake between rovers and sitters. (B) Re-feeding experiments show that after three hours of acute food deprivation, followed by 0, 30 min, 1 h or 2 h of re-feeding, food intake levels in forR larvae return to their initial well-fed food intake levels more slowly than in fors and fors2 larvae. (C) Hemolymph carbohydrate levels in forR larvae returned to well-fed levels after 2 h of re-feeding, whereas hemolymph carbohydrate levels in fors and fors2 were not changed by acute food deprivation (see Fig. 2D) or re-feeding. (D) Well-fed forR larvae have significantly lower akh mRNA levels than fors and fors2. Relative akh mRNA levels were measured using quantitative real-time PCR and standardized against forR larvae. Error bars indicate means ± s.e.m.

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