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First published online July 14, 2008
Journal of Experimental Biology 211, 2542-2550 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.015586
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Post-prandial alkaline tide in freshwater rainbow trout: effects of meal anticipation on recovery from acid–base and ion regulatory disturbances

C. A. Cooper* and R. W. Wilson

School of Biosciences, University of Exeter, Hatherly Laboratories, Exeter, Devon EX4 4PS, UK


Figure 1
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Fig. 1. The change in (A) blood pH (solid line), plasma HCO3 (mmol l–1; dashed line), and (B) the partial pressure of CO2 (PCO2) over the duration of the experiment in which fish were fed a 1% of body mass ration via an oesophageal catheter. Values are means ± s.e.m. (N=11). An asterisk denotes a significant difference when compared with pre-feed levels [adjusted P<0.05; RM-ANOVA followed by a multiple pairwise control (pre-feed) comparisons versus post-feed groups using the Bonferroni t-test method], whereas a dagger indicates a significant difference of {Delta}pH when compared with voluntarily fed fish (P<0.05; paired t-test).

 

Figure 2
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Fig. 2. Plasma concentrations of (A) chloride (Cl, dashed line), sodium (Na+, solid line), (B) potassium (K+, solid line), calcium (Ca2+, dashed line) and magnesium (Mg2+, dotted line), over the duration of the experiment in which fish were fed a 1% of body mass ration via an oesophageal catheter. Values are means ± s.e.m. (N=11). A single asterisk denotes P<0.05, whereas a double asterisk indicates P<0.01 [adjusted P value; RM-ANOVA followed by a multiple pairwise control (pre-feed) comparisons versus post-feed groups using the Bonferroni t-test method].

 

Figure 3
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Fig. 3. Fluxes of titratable alkalinity (JTAlk, grey bars), total ammonia (JTamm, white bars) and net acidic equivalents (JnetH+ black bars) over a 72 h period after fish were fed a 1% of body mass ration via an oesophageal catheter. Positive values indicate base excretion and negative values indicate acid excretion. Values are means ± s.e.m. (N=8–11). An asterisk denotes a significant difference when compared with pre-feed levels [adjusted P<0.05; RM-ANOVA followed by a multiple pairwise control (pre-feed) comparisons versus post-feed groups using the Bonferroni t-test method], whereas a dagger indicates a significant difference of {Delta}JTAlk or JTamm when compared with voluntarily fed fish (P<0.05; paired t-test).

 

Figure 4
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Fig. 4. The change in (A) blood pH (solid line), plasma HCO3 (mmol l–1, dashed line) and (B) the partial pressure of CO2 (PCO2), over the duration of the experiment when fish fed voluntarily on a 1% of body mass ration. Values are means ± s.e.m. (N=8). An asterisk denotes a significant difference when compared with pre-feed levels [adjusted P<0.05; RM-ANOVA followed by a multiple pairwise control (pre-feed) comparisons versus post-feed groups using the Bonferroni t-test method], whereas a dagger indicates a significant difference in {Delta}pH when compared with catheter-fed fish (P<0.05; paired t-test).

 

Figure 5
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Fig. 5. Plasma concentrations of (A) chloride (Cl, solid line), sodium (Na+, dashed line), (B) potassium (K+, solid line), calcium (Ca2+, dashed line) and magnesium (Mg2+, dotted line), over the duration of the experiment when fish were allowed to feed voluntarily on a 1% of body mass ration. Values are means ± s.e.m. (N=8).

 

Figure 6
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Fig. 6. Fluxes of titratable alkalinity (JTAlk, grey bars), total ammonia (JTamm, white bars) and net acidic equivalents (JnetH+ black bars) over a 72 h period after fish fed voluntarily on a 1% of body mass ration. Positive values indicate base excretion and negative values indicate acid excretion. Values are means ± s.e.m. (N=8). An asterisk denotes a significant difference when compared with pre-feed levels (adjusted P<0.05; RM-ANOVA followed by a multiple pairwise control (pre-feed) comparisons versus post-feed groups using the Bonferroni t-test method), whereas a dagger indicates a significant difference of {Delta}JTAlk and JTamm when compared with voluntarily fed fish (P<0.05; paired t-test).

 

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© The Company of Biologists Ltd 2008