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First published online June 13, 2008
Journal of Experimental Biology 211, 2172-2184 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.016592

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Expression of myogenic regulatory factors in the muscle-derived electric organ of Sternopygus macrurus

Jung A. Kim^1,*, Christine Laney¹, Jeanne Curry² and Graciela A. Unguez^1,

¹ Department of Biology, New Mexico State University, Las Cruces, NM 88003, USA
² Department of Agronomy and Horticulture, New Mexico State University, Las Cruces, NM 88003, USA

View larger version (70K): [in this window] [in a new window]   Fig. 1. Sequence comparison of the deduced proteins encoded by S. macrurus myf5 with comparable sequences from mouse (A) and other piscine species (B). Protein sequences from mouse (M. musculus, GenBank accession number NP_032682.1), carp (C. carpio, GenBank accession number BAA33566), zebrafish (D. rerio, GenBank accession number NP_571651.1), striped seabass (M. saxitilis, GenBank accession number AF463525_1), rainbow trout (O. mykiss, GenBank accession number AAV30214) and pufferfish (T. rubripes, GenBank accession number CAC39208.1) were used. The boxed areas are labeled to indicate the N-terminal, His/Cys-rich, basic helix-loop-helix (bHLH) and helix III domains. Asterisks represent amino acid similarities across sequences.

View larger version (52K): [in this window] [in a new window]   Fig. 2. Sequence comparison of the deduced protein encoded by S. macrurus MRF4 with comparable sequences from mouse (A) and other piscine species (B). Sequences from mouse (M. musculus, GenBank accession number 1604247A), zebrafish (D. rerio, GenBank accession number AAQ67704) and puffer fish (T. rubripes, GenBank accession number AAR20812) were used. The boxed areas are labeled to indicate the N-terminal, the His/Cys-rich, bHLH and Helix III domains. Asterisks represent amino acid similarities across sequences.

View larger version (63K): [in this window] [in a new window]   Fig. 3. Expression of MyoD, myogenin, myf5 and MRF4 transcripts in adult tissues of S. macrurus by RT-PCR analysis. Total RNAs from skeletal muscle, EO, liver and brain were reverse transcribed and used for PCR. PCR products (1.0 µg per lane) were resolved on agarose gels containing ethidium bromide, and the resultant bands are presented as negative images of the original gels. Partial cDNA fragments of MyoD (290 bp), myogenin (312 bp), myf5 (209 bp) and MRF4 (301 bp) were detected in skeletal muscle and EO, but not in liver or brain. Control reactions without reverse transcriptase (No RT lanes) were carried out for muscle (lane 2), EO (lane 4), liver (lane 6) and brain (lane 8) to ensure that PCR products were RNA dependent and not the result of genomic DNA amplification. RT-PCR analysis of glyceraldehyde dehydrogenase (GAPDH) was used as a loading control. Lanes labelled M represent the 1 kb+ DNA ladder.

View larger version (11K): [in this window] [in a new window]   Fig. 4. Expression of MyoD, myogenin, myf5 and MRF4 transcripts in muscle and EO from control fish (N=5) and fish after 5 weeks spinal transection (ST, N=5) by quantitative RT-PCR. The amount of MyoD, myogenin, myf5 and MRF4 transcript per tissue type was obtained from real-time RT-PCR experiments, and data are represented as molecules of transcript per µl of mRNA of each tissue type. Each column represents the mean ± s.e.m. Significantly different from control; *significantly different from control or 5 week ST muscle groups at P0.05.

View larger version (71K): [in this window] [in a new window]   Fig. 5. Expression of Id1, Id2 and MEF2C in adult tissues of S. macrurus by RT-PCR. Agarose gel showing the expression of partial Id1 (304 bp), Id2 (101 bp) and MEF2C (265 bp) cDNA fragments in skeletal muscle, EO, liver, brain and heart of S. macrurus using RT-PCR. For each transcript, 1 µg of cDNA was used for the PCR amplification. RT-PCR analysis of GAPDH was used as a loading control. Lanes labeled M represent the 1 kb+ or 25 bp (Id2) DNA ladder. Other lanes as in Fig. 3.

View larger version (74K): [in this window] [in a new window]   Fig. 6. Expression of Id1, Id2 and MEF2C in adult and 5 week ST muscle and EO by RT-PCR. Agarose gel showing the expression of Id1, Id2 and MEF2C in skeletal muscle and EO from control un-operated and 5 week ST fish using RT-PCR. Id1, Id2 and MEF2C transcripts were detected in all tissues analyzed. The size of the cDNA fragments for each transcript was the same as that in Fig. 5; 1 µg of cDNA was used for PCR amplification. RT-PCR analysis of GAPDH was used as a loading control. Lanes labeled M represent the 1 kb+ or 25 bp (Id2) DNA ladder. Other lanes as in Fig. 3.

View larger version (36K): [in this window] [in a new window]   Fig. 7. Genomic Southern blot analysis of the MyoD, myogenin, myf5 and MRF4 genes. Genomic DNA prepared from S. macrurus adults was digested with BamHI (lane 1), EcoRI (lane 2), HindIII (lane 3), BamHI/EcoRI (lane 4), BamHI/HindIII (lane 5) and EcoRI/HindIII (lane 6), resolved by 1% agarose gel electrophoresis, and transferred to a nylon membrane. Genomic DNA was loaded at 5 µg per lane. Blots were hybridized with 32P-labeled probes specific to S. macrurus MyoD, myogenin, myf5 and MRF4, and washed under high stringency conditions.

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