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First published online May 30, 2008
Journal of Experimental Biology 211, 1919-1926 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.013748
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Evaluation of thyroid-mediated otolith growth of larval and juvenile tilapia

Jen-Chieh Shiao1,*, Su-Mei Wu2,*, Yi-Ping Hwang2, Done-Ping Wu3 and Pung-Pung Hwang4,{dagger}

1 Institute of Oceanography, College of Science, National Taiwan University, Taipei, Taiwan, Republic of China
2 Department of Aquatic Biosciences, College of Life Science, National Chiayi University, Chiayi, Taiwan, Republic of China
3 St Martin De Porres Hospital, Chiayi, Taiwan, Republic of China
4 Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan, Republic of China


Figure 1
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  		Fig. 1. Temporal change in T3 content (N=5, open circles) and otolith growth (N=14, filled circles) of tilapia larvae and juveniles during the experimental period. T3 content was measured every 2 days but samples at 27 day post-hatching were lost. The data are given as means ± s.e.m. for clarity.

 

Figure 2
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  		Fig. 2. Ontogenic expression of thyroid hormone receptor {alpha} (filled circles) and β (open circles) RNA during tilapia larval and juvenile stages (N=3). The mRNA expression was normalized in relation to β-actin. The data are given as means ± s.e.m.

 

Figure 3
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  		Fig. 3. Microstructure and morphology of otoliths from tilapia juveniles immersed in 300 p.p.m. thiourea (TU) from 15 to 27 days post-hatching (d.p.h.), followed by a recovery period in fresh water from 28 to 41 d.p.h. (C,D,F). Otoliths of normal fish are shown in A,B,E for comparison. The innermost fluorescent ring indicates the beginning of TU treatment. The outer three fluorescent rings were laid down on the second, fourth and sixth day after ending TU treatment. A–D are left sagittae; E and F are right sagittae. AR, antirostrum; PR, postrostrum; R, rostrum. Scale bars, 100 µm.

 

Figure 4
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  		Fig. 4. Otolith growth rate during the 300 p.p.m. thiourea (TU) treatment from 15 to 27 d.p.h., followed by recovery in fresh water from 28–41 d.p.h. Filled circles indicate the control group without treatment; open circles indicate the experimental group (N=12 for each group). Data are shown as means ± s.e.m. for clarity. Arrows indicate the dates of treatment with tetracycline that produced the fluorescent rings as shown in Fig. 3.

 

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© The Company of Biologists Ltd 2008