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Fig. 1. Upstream regions of Gyc-89Da and Gyc-89Db drive green
fluorescent protein (GFP) expression in sensory neurons in larvae in a similar
pattern to that seen with in situ hybridization for Gyc-89Da
and Gyc-89Db in embryos. Second instar larvae containing the
p89Da-GFP (B,E,H,K) or p89Db-GFP (C,F,I,L) transgenes were
examined for GFP fluorescence and compared with embryos stained with a
digoxigenin-labeled riboprobe for Gyc-89Db (A,D,G,J). Anterior is to
the left (A–C) or up (D–L). (A–C) Low magnification showing
the three populations of sensory neurons stained: chemosensory neurons in the
dorsal ganglion (DG) and terminal ganglion (TG), external sensilla (ES)
neurons and neurons in the terminal sensory organs (SO). (D–F) High
magnification of the lateral body wall showing a neuron innervating the
external sensilla. (G–I) High magnification of the anterior end of
embryo or larvae showing neurons in the dorsal ganglion and terminal ganglion
that innervate the dorsal organ (DO) and terminal organ, respectively.
p89Db-GFP also drives GFP expression in neurons in the
monoscolopidial organ (mc). (J–L) High magnification of the posterior
end of embryo or larvae showing a single neuron innervating each terminal
sensory organ. Fluorescence seen in tracheae is due to autofluorescence. Scale
bars represent 200 µm (A), 500 µm (B,C), 50 µm (D,G,J) and 100 µm
(E,F,H,I,K,L).
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