First published online April 20, 2007
Journal of Experimental Biology 210, 1593-1601 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.000141
Functional genomics and proteomics of the cellular osmotic stress response in `non-model' organisms
Dietmar Kültz1,*,
Diego Fiol1,
Nelly Valkova1,
Silvia Gomez-Jimenez2,
Stephanie Y. Chan1 and
Jinoo Lee1
1 Physiological Genomics Group, Department of Animal Science, One Shields
Avenue, University of California, Davis, CA 95616, USA
2 Laboratorio de Fisiología de Invertebrados Marinos, CIAD, A.C.
Carr. a la Victoria Km. 0.6, CP 83000, Hermosillo, Sonora,
México

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Fig. 1. Example of a time course of mRNA expression of SSH clones in response to
salinity stress (SSH#81, protein phsophatase-2A catalytic subunit, is shown).
mRNA expression was analyzed at the indicated times after transfer of tilapia
from freshwater to seawater (SW). Expression levels were quantified by qPCR in
gill epithelial cells. Values are means ± s.e.m. (N=4).
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Fig. 2. Tetilla mutabilis proteome map, with proteins that were picked for
mass spectrometric analysis labeled. Red labels denote proteins that are
upregulated during emersion or post-emersion stress, and blue labels denote
proteins that are unchanged by emersion stress. The vertical dimension of the
2-D gel represents the molecular mass scale (10120 kDa on 11% uniform
polyacrylamide gels), and the horizontal dimension represents the isoelectric
point scale (pH 310 on non-linear 24 cm IPG strips).
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© The Company of Biologists Ltd 2007