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First published online April 20, 2007
Journal of Experimental Biology 210, 1593-1601 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.000141
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Functional genomics and proteomics of the cellular osmotic stress response in `non-model' organisms

Dietmar Kültz1,*, Diego Fiol1, Nelly Valkova1, Silvia Gomez-Jimenez2, Stephanie Y. Chan1 and Jinoo Lee1

1 Physiological Genomics Group, Department of Animal Science, One Shields Avenue, University of California, Davis, CA 95616, USA
2 Laboratorio de Fisiología de Invertebrados Marinos, CIAD, A.C. Carr. a la Victoria Km. 0.6, CP 83000, Hermosillo, Sonora, México


Figure 1
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  		Fig. 1. Example of a time course of mRNA expression of SSH clones in response to salinity stress (SSH#81, protein phsophatase-2A catalytic subunit, is shown). mRNA expression was analyzed at the indicated times after transfer of tilapia from freshwater to seawater (SW). Expression levels were quantified by qPCR in gill epithelial cells. Values are means ± s.e.m. (N=4).

 

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  		Fig. 2. Tetilla mutabilis proteome map, with proteins that were picked for mass spectrometric analysis labeled. Red labels denote proteins that are upregulated during emersion or post-emersion stress, and blue labels denote proteins that are unchanged by emersion stress. The vertical dimension of the 2-D gel represents the molecular mass scale (10–120 kDa on 11% uniform polyacrylamide gels), and the horizontal dimension represents the isoelectric point scale (pH 3–10 on non-linear 24 cm IPG strips).

 




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