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First published online March 31, 2007
Journal of Experimental Biology 210, 1303-1310 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.003418
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Osmoregulation in elephant fish Callorhinchus milii (Holocephali), with special reference to the rectal gland

Susumu Hyodo1,*, Justin D. Bell2,3, Jillian M. Healy2, Toyoji Kaneko4, Sanae Hasegawa1, Yoshio Takei1, John A. Donald2 and Tes Toop2

1 Laboratory of Physiology, Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo 164-8639, Japan
2 School of Life and Environmental Sciences, Deakin University, Victoria, Australia
3 Primary Industries Research Victoria, Queenscliff, Victoria, Australia
4 Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo, Tokyo, Japan


Figure 1
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  		Fig. 1. Gross anatomy of the rectal part of the intestine in elephant fish (A–C) and Southern chimaera (D,E). (A) A longitudinal slice of the post-valvular intestine. The arrow indicates the anterior end of glandular tissue at the termination of valvular intestine. (B,D) Whole-mount view of the glandular tissue of elephant fish (B) and Southern chimaera (D). Luminal epithelia and inner muscle layers were removed to expose the glandular tissue. Arrows in D indicate openings of the glandular tissues. (C,E) Magnified view of the glandular tissue in B and D, respectively. Scale bars, 0.5 mm (A); 1 mm (B,D); 200 µm (C,E).

 

Figure 2
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  		Fig. 2. Sagittal sections of the glandular structures in elephant fish (A,B) and Southern chimaera (C). Sections were stained with anti-Na+,K+-ATPase serum (A,C) and with periodic acid–Schiff (PAS; B). Asterisks in A and B indicate a central duct that runs along the anteroposterior axis. Scale bar, 1 mm.

 

Figure 3
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  		Fig. 3. Cross sections of the glandular structures in the rectal part of the intestine in elephant fish. Adjacent sections were stained with anti-Na+,K+-ATPase serum (A,C,D) and with periodic acid–Schiff (PAS; B). (C,D) Magnified views of the section in A, showing the transition from the secretory epithelium (stained with anti-Na+,K+-ATPase serum) to the mucus cells along the single tubule (arrows). Immmunoreactive signals for Na+,K+-ATPase were localized on the basolateral membranes but not on the apical membranes of epithelial cells. Scale bars, 500 µm (A–C); 100 µm (D).

 

Figure 4
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  		Fig. 4. A series of cross sectional views of the posterior portion of the glandular tissue from the rostral (A) to caudal (C), showing openings of the glandular structures into the lumen of the intestine (arrows). Asterisks indicate central ducts. Scale bar, 500 µm.

 




© The Company of Biologists Ltd 2007