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First published online March 2, 2007
Journal of Experimental Biology 210, 956-963 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.001586
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The role of larval fat cells in adult Drosophila melanogaster

Jerell R. Aguila1, Justin Suszko1,*, Allen G. Gibbs1 and Deborah K. Hoshizaki1,2,{dagger}

1 School of Life Sciences, University of Nevada Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154, USA
2 UNLV Institute for Cell and Molecular Medicine, University of Nevada Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154, USA


Figure 1
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  		Fig. 1. Starvation resistance of y w adult flies decreases with age. Starvation resistance was measured by percentage survival of adult females in groups of 10 flies. Newly eclosed y w adults (N=20 groups of 10) (squares), 3-day-old y w adults (N=30 groups of 10) (diamonds), 10-day-old y w adults (N=10 groups of 10) (triangles). Values are means ± s.d.

 

Figure 2
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  		Fig. 2. Free-floating fat cells in the adult are dissociated larval fat body cells. Free-floating fat cells from an adult labeled with a polytene chromosome GFP cell marker (G000343/CyO). Scale bar, 200 µm.

 

Figure 3
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  		Fig. 3. Whole-mount adults used for GFP-based measurement of larval fat cells. (A–C) Fluorescent images of whole-mount Lsp2-Gal4::UAS-GFP aged adult females. GFP-labeled larval fat cells are prominent in the abdomen. (D) Phosphoimage of whole-mount Lsp2-Gal4::UAS-GFP aged adult females used to quantify larval fat cells.

 

Figure 4
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  		Fig. 4. GFP fluorescence in adults is directly related to in situ percentage fat-cell number. Larval fat-cell number for Lsp2-Gal4::UAS-GFP adult females using the GFP-based assay (N=44–60 individuals per time point) compared with in situ fat-cell numbers from dissected individual females (N=28–46 individuals per time point). Values are means ± s.d. Squares, percentage fluorescence; diamonds, percentage cell number.

 

Figure 5
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  		Fig. 5. Starvation resistance of Lsp2-Gal4::UAS-GFP adults decreases with age. Starvation resistance was measured by percentage survival of adult females in groups of 10 flies. Newly eclosed Lsp2-Gal4::UAS-GFP adults (N=8 groups of 10) (squares), 3-day-old Lsp2-Gal4::UAS-GFP adults (N=14 groups of 10) (diamonds), 10-day-old Lsp2-Gal4::UAS-GFP adults (N=10 groups of 10) (triangles). Values are means ± s.d.

 

Figure 6
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  		Fig. 6. Larval fat-cell number and starvation resistance in newly eclosed adults. Larval fat-cell number measured for Lsp2-Gal4::UAS-GFP adult females using the GFP-based assay (N=25–36 per time point), and compared with the percentage survival of newly eclosed Lsp2-Gal4::UAS-GFP starved adult females (N=8 groups of 10). Diamonds, percentage fluorescence; squares, percentage survival. Values are means ± s.d.

 

Figure 7
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  		Fig. 7. Larval fat cells persist in aged adults when cell death is blocked. (A) In situ fat-cell number from Lsp2-Gal4::UAS-GFP/UAS-diap1 adult females in which cell death is blocked (N=15–20 individuals per time point, filled bar) compared with Lsp2-Gal4::UAS-GFP control adult females (N=10–46 individuals per time point, open bar). (Mean initial cell number for Lsp2-Gal4::UAS-GFP/UAS-diap1 was 792 cells; for Lsp2-Gal4::UAS-GFP it was 724 cells.) (B) GFP-fluorescence of Lsp2-Gal4::UAS-GFP/UAS-diap1 adult females (N=15–25 per time point, filled bar). Lsp2-Gal4::UAS-GFP control adult females (N=10–60 individuals per time point, open bar). (Mean initial fluorescence for Lsp2-Gal4::UAS-GFP/UAS-diap1 was 25 600 pixels; for Lsp2-Gal4::UAS-GFP it was 21 900 pixels.) Note, perdurance of GFP-fluorescence does not reflect fat-cell number in the cell death-blocked animals. This is probably because of a loss of activity from the Lsp2 promoter (see Discussion for details). Values are means ± s.d.

 

Figure 8
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  		Fig. 8. Starvation resistance increases in adults carrying larval fat cells in which cell death is blocked. Starvation resistance was measured by percentage survival of newly eclosed adult females in groups of 10 individuals. Control, Lsp2-Gal4::UAS-GFP adults (N=80 groups of 10, squares). Larval fat cells with extended lifespan, Lsp2-Gal4::UAS-GFP/UAS-p35 adults (N=20 groups of 10, diamonds), Lsp2-Gal4::UAS-GFP/UAS-diap1 adults (N=20 groups of 10, circles), and UAS-diap1/+; Lsp2-Gal4::UAS-GFP/+ adults (N=20 groups of 10, triangles). Values are means ± s.d.

 

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© The Company of Biologists Ltd 2007