First published online January 31, 2007
Journal of Experimental Biology 210, 620-627 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.02684
V-type H+-ATPase and Na+,K+-ATPase in the gills of 13 euryhaline crabs during salinity acclimation
Jyuan-Ru Tsai1 and
Hui-Chen Lin1,2,*
1 Department of Life Science, Tunghai University, Taichung 40704, Taiwan
2 Center for Tropical Ecology and Biodiversity, Tunghai University, Taichung
40704, Taiwan

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Fig. 1. Typical immunohistochemical staining in gill epithelia. (A)
Macrophthalmus abbreviatus; red deposits represent a cytoplasmic HA
distribution. (B) Macrophthalmus abbreviatus; negative control. (C)
Eriocheir sinensis; red deposits represent an apical HA distribution.
(D) Eriocheir sinensis; negative control. A, apical region. C,
cytoplasm. cu, cuticle. n, nucleus. p, pillar cell. se, septum. Bars, 20
µm.
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Fig. 2. (A) Uca formosensis. V-type H+-ATPase activity in 5
p.p.t. and 35 p.p.t. salinity. *P=0.01. (B) U. formosensis.
Na+,K+-ATPase activity in 5 p.p.t. and 35 p.p.t.
salinity. There was no significant difference between salinities.
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Fig. 3. Relative protein abundance of V-type H+-ATPase in U.
formosensis. There was no statistical difference between salinity
treatments.
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© The Company of Biologists Ltd 2007