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First published online January 17, 2007
Journal of Experimental Biology 210, 477-483 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.02665

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Effect of maternal myostatin antibody on offspring growth performance and body composition in mice

Yu-Chuan Liang¹, Jan-Ying Yeh² and Bor-Rung Ou^1,3,*

¹ Department of Animal Science and Biotechnology, Tunghai University, Taichung 407, Taiwan, Republic of China,
² Department of Biotechnology and Bioinformatics, Asia University, Wufeng Shiang, Taichung 413, Taiwan, Republic of China
³ Life Science Research Center, Tunghai University, Taichung 407, Taiwan, Republic of China

View larger version (11K): [in this window] [in a new window]   Fig. 1. Determination of maternal myostatin antibody titer. (A) Absorbance at 415 nm by myostatin-ELISA with a twofold series dilution of serum from 1:320. Each data point represents the mean of five mice. (B) Dot blotting analysis of maternal myostatin Ab with two-fold series serum dilution from 1:800.

View larger version (56K): [in this window] [in a new window]   Fig. 2. Effect of maternal myostatin antibody on embryo development in mice. (A) Amount of collected embryo between mstn Ab-induced and control groups. Values are means ± s.d. (N=6). Asterisks indicate a significant difference compared with control (P<0.05). (B) Collected embryo from control group and (C) embryo after overnight culture. (D) Collected embryo from mstn Ab-induced mice and (E) after overnight culture.

View larger version (8K): [in this window] [in a new window]   Fig. 3. Histological stain of mouse ovary from control group and mstn Ab-induced group. The number of (A) primordial and (B) developing follicles was determined in each fifth section of a series of 5 mm sections as described in Materials and methods. Values are means ± s.d. (N=6). Asterisks indicate a significant difference compared with control (P<0.05).

View larger version (6K): [in this window] [in a new window]   Fig. 4. Titer determination of myostatin Ab in offspring mice by myostatin-ELISA with a twofold series dilution of serum from 1:10. Each data point represents the mean of five mice ± s.d.

View larger version (7K): [in this window] [in a new window]   Fig. 5. Effect of maternal myostatin Ab on growth performance in (A) male and (B) female offspring. Values are means ± s.d. Body masses were measured from male control mice (N=33), male maternal mstn Ab-induced mice (N=13), female control mice (N=31) and female maternal mstn Ab-induced mice (N=13). Asterisks indicate a significant difference in body mass between mstn Ab-induced and control groups at the same age (P<0.05).

View larger version (11K): [in this window] [in a new window]   Fig. 6. Effect of maternal myostatin Ab on body composition of offspring. (A) Protein, (B) fat and (C) water were measured from male control mice (N=33), male maternal mstn Ab-induced mice (N=13), female control mice (N=31) and female maternal mstn Ab-induced mice (N=13). Data represent means ± s.d. Asterisks indicate a significant difference between mstn Ab-induced and control groups of the same gender (P<0.05).