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First published online August 9, 2007
Journal of Experimental Biology 210, 2885-2896 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.002873
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Differential expression of gill Na+,K+-ATPase {alpha}- and ß-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar

Tom O. Nilsen1,*, Lars O. E. Ebbesson1, Steffen S. Madsen2, Stephen D. McCormick3, Eva Andersson4, Björn Th. Björnsson5, Patrick Prunet6 and Sigurd O. Stefansson1

1 Department of Biology, University of Bergen, High Technology Centre, Bergen N-5020, Norway
2 Institute of Biology, University of Southern Denmark, DK-5230 Odense M, Denmark
3 USGS, Conte Anadromous Fish Research Center, Turners Falls, MA 01376, USA
4 Institute of Marine Research, Nordnes N-5817 Norway
5 Fish Endocrinology Laboratory, Department of Zoology/Zoophysiology, Göteborg University, Box 463, S40530, Göteborg, Sweden
6 INRA-SCRIBE, Fish Adaptation and Stress Group, IFR Reproduction, Development and Ecophysiology, Rennes Cedex, France


Figure 1
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  		Fig. 1. (A) Gill Na+, K+-ATPase activity in anadromous (closed circles) and landlocked (open circles) Atlantic salmon in FW and SW from February 26 through June 18; (B) plasma [Cl-] in SW from March 9 through June 22. Symbols for anadromous (closed triangles) and landlocked salmon (open triangles) after 96 h SW (34%thou) exposure, and anadromous (closed diamonds) and landlocked salmon (open diamonds) in mid-June after 1 month in SW are offset for clarity. Values are means ± s.e.m. (N=8-10). *Significant difference between strains in FW (P<0.05). Different capital and small letters denote differences (P<0.05) between time points within anadromous and landlocked salmon, respectively. {dagger}Significant differences between FW and SW in landlocked salmon.

 

Figure 2
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  		Fig. 2. Gill Na+, K+-ATPase {alpha}1a (A) and {alpha}1b (B) mRNA levels in anadromous (closed circles) and landlocked (open circles) Atlantic salmon in FW from February 26 through June 18. Symbols for anadromous (closed triangle) and landlocked salmon (open triangle) after 96 h SW (34%thou) exposure, and anadromous (closed diamond) and landlocked salmon (open diamond) in mid-June after 1 month in SW are offset for clarity. Values are means ± s.e.m. (N=8-10). *Significant difference between strains in FW (P<0.05). Different capital and small letters denote differences (P<0.05) between time points within anadromous and landlocked salmon, respectively. Significant differences between FW and SW are indicated by {dagger}(landlocked) {ddagger}(anadromous) salmon.

 

Figure 3
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  		Fig. 3. Gill Na+, K+-ATPase {alpha}3 (A) and Na+, K+-ATPase ß1 (B) mRNA levels in anadromous (closed circles) and landlocked (open circles) Atlantic salmon in FW from February 26 through June 18. Symbols for anadromous (closed triangle) and landlocked salmon (open triangle) after 96 h SW (34%thou) exposure, and anadromous (closed diamond) and landlocked salmon (open diamond) in mid-June after 1 month in SW are offset for clarity. Values are means ± s.e.m. (N=8-10). *Significant difference between strains in FW (P<0.05). Different capital and small letters denote differences (P<0.05) between time points within anadromous and landlocked salmon, respectively. Significant differences between FW and SW are indicated by {dagger}(landlocked) {ddagger}(anadromous) salmon.

 

Figure 4
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  		Fig. 4. Gill Na+, K+-ATPase {alpha}-protein abundance in anadromous (closed circles) and landlocked (open circles) Atlantic salmon in FW from February 26 through June 18. Symbols for anadromous (closed diamond) and landlocked salmon (open diamond) in mid-June after 1 month in SW are offset for clarity. Values are means ± s.e.m. (N=5-6). Different capital and small letters denote differences (P<0.05) between time points within anadromous and landlocked salmon, respectively. Significant differences between FW and SW are indicated by {dagger} (landlocked) and {ddagger} (anadromous) salmon.

 

Figure 5
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  		Fig. 5. Gill Na+ K+, 2Cl- cotransporter mRNA levels (A) and protein abundance (B in anadromous (closed circles) and landlocked (open circles) Atlantic salmon in FW from February 26 through June 18. Symbols for anadromous (closed triangle) and landlocked salmon (open triangle) after 96 h SW (34%thou) exposure, and anadromous (closed diamond) and landlocked salmon (open diamond) in mid-June after 1 month in SW are offset for clarity. Values are means ± s.e.m. (N=8-10). *Significant difference between strains in FW (P<0.05). Different capital and small letters denote differences (P<0.05) between timepoints within anadromous and landlocked salmon, respectively. {dagger}Significant differences between FW and SW in landlocked salmon.

 

Figure 6
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  		Fig. 6. Gill cystic fibrosis transmembrane conductance regulator I (CFTR I) mRNA levels in anadromous (closed circles) and landlocked (open circles) Atlantic salmon in FW from February 26 through June 18. CFTR I was not measured following SW transfer. Values are means ± s.e.m. (N=8-10). *Significant difference between strains in FW (P<0.05). Different capital and small letters denote differences (P<0.05) between time points within anadromous and landlocked salmon, respectively.

 

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© The Company of Biologists Ltd 2007