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First published online June 15, 2007
Journal of Experimental Biology 210, 2346-2351 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.002857

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Physiological regulation of glucose transporter (GLUT4) protein content in brown trout (Salmo trutta) skeletal muscle

Mònica Díaz, Encarnación Capilla^* and Josep V. Planas

Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain

View larger version (8K): [in this window] [in a new window]   Fig. 1. Total protein content of btGLUT4 in red and white muscle from brown trout. (A) Immunodetection of btGLUT4 and actin protein in total membrane preparations from red and white muscle. 25 µg of protein was loaded in each lane, subjected to SDS-PAGE and immunoblotted using antibodies against okGLUT4 and chicken actin. (B) Densitometric analysis of the btGLUT4/actin ratios from red muscle and white muscle. Values are means ± s.e.m. and are referred to red muscle, which was set to 1 (N=6). *Significant differences compared with red muscle (P<0.01).

View larger version (11K): [in this window] [in a new window]   Fig. 2. Effects of fasting (45 days) on the btGLUT4 protein content of red muscle and white muscle from brown trout. (A) Immunodetection of btGLUT4 and actin protein in total membrane preparations from red and white muscle. 25 µg of protein was loaded in each lane, subjected to SDS-PAGE and immunoblotted using antibodies against okGLUT4 and chicken actin. (B) Densitometric analysis of the btGLUT4/actin ratios from red muscle and white muscle. Values are means ± s.e.m. and are referred to the fed group (control), which was set to 1 (N=4-5). *Significant differences compared with the control group for each type of muscle (P<0.01).

View larger version (12K): [in this window] [in a new window]   Fig. 3. Effects of insulin treatment (24 h) on the btGLUT4 protein content of red muscle and white muscle from brown trout. (A) Immunodetection of btGLUT4 and actin protein in total membrane preparations from red and white muscle. 25 µg of protein was loaded in each lane, subjected to SDS-PAGE and immunoblotted using antibodies against okGLUT4 and chicken actin. (B) Densitometric analysis of the btGLUT4/actin ratios from red muscle and white muscle. Values are means ± s.e.m. and are referred to the saline-injected group, which was set to 1 (N=5-6). *Significant differences compared with the saline-injected group for each type of muscle (P<0.05).

View larger version (13K): [in this window] [in a new window]   Fig. 4. Effects of arginine treatment (24 h) on the btGLUT4 protein content of red muscle and white muscle from brown trout. (A) Immunodetection of btGLUT4 and actin protein in total membrane preparations from red and white muscle. 25 µg of protein was loaded in each lane, subjected to SDS-PAGE and immunoblotted using antibodies against okGLUT4 and chicken actin. A representative western blot is shown. (B) Densitometric analysis of the btGLUT4/actin ratios from red muscle and white muscle. Values are means ± s.e.m. and are referred to the saline-injected group, which was set to 1 (N=10).

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