spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online June 11, 2007
Journal of Experimental Biology 210, 2137-2145 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.006098
This Article
Right arrow Summary Freely available
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Werneke, S. W.
Right arrow Articles by Smith, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Werneke, S. W.
Right arrow Articles by Smith, A. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

The role of metals in molluscan adhesive gels

S. W. Werneke, C. Swann, L. A. Farquharson, K. S. Hamilton and A. M. Smith*

Department of Biology, Ithaca College, Ithaca, NY 14850, USA


Figure 1
View larger version (3K):
[in this window]
[in a new window]

  		Fig. 1. The metal content of the glue of A. subfuscus for three samples using the longest hydrolysis, as determined by the ICP atomic absorption spectrometer. There was no detectable cobalt or nickel. Values are mean ± s.e.m.

 

Figure 2
View larger version (20K):
[in this window]
[in a new window]

  		Fig. 2. Electroblot showing the iron-binding ability of proteins in the glue of A. subfuscus. The left side shows the Coomassie Blue-stained gel and the right side shows the corresponding ferene-stained membrane. Bovine serum albumin (BSA) is in the left lane of each as a control, whereas the dissolved glue is in the right lane. Molecular masses are indicated in the left margin.

 

Figure 3
View larger version (26K):
[in this window]
[in a new window]

  		Fig. 3. The effect of specific ions on the proteins in the adhesive gel from A. subfuscus. The same amount of dissolved glue was initially present in each sample. From left to right, the lanes had either 10 mmol l–1 FeCl3, 1 mmol l–1 EDTA, 10 mmol l–1 MgCl2, 10 mmol l–1 CaCl2 or no extra ions added. Molecular masses are indicated in the left margin.

 

Figure 4
View larger version (9K):
[in this window]
[in a new window]

  		Fig. 4. The effect of deferoxamine on the solubility of A. subfuscus glue. Bars show the relative staining intensity of three primary groups of bands on the gel. Proteins were extracted by either a neutral Tris buffer or the same buffer with deferoxamine. (A) Extraction of proteins from gels that have already set. (B) Extraction of proteins from gels as they are being secreted (during setting).

 

Figure 5
View larger version (5K):
[in this window]
[in a new window]

  		Fig. 5. The effect of iron on the ability of A. subfuscus glue proteins to stiffen 2% citrus pectin. Stiffness (storage modulus) was measured with a dynamic rheometer. Approximately 0.5 mg ml–1 total protein was used from the glue, and 1 mmol l–1 deferoxamine (defx).

 

Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?



© The Company of Biologists Ltd 2007