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First published online December 14, 2006
Journal of Experimental Biology 210, 149-165 (2007)
Published by The Company of Biologists 2007
doi: 10.1242/jeb.02628
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Changes in mitochondrial oxidative capacities during thermal acclimation of rainbow trout Oncorhynchus mykiss: roles of membrane proteins, phospholipids and their fatty acid compositions

Edouard Kraffe1,2,*, Yanic Marty1 and Helga Guderley2

1 Unité mixte CNRS 6521, Université de Bretagne Occidentale, C.S. 93837, 29238 Brest cedex 3, France
2 Département de Biologie, Université Laval, Québec, G1K 7P4, Canada


Figure 1
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  		Fig. 1. Concentration of mitochondrial cytochromes in oxidative muscle during warm acclimation. Cytochrome and ANT concentrations are normalised to the protein content in the mitochondrial preparations. Values are means ± s.e.m. (N=9 for cold acclimated trout, N=8 for short-term warm exposed trout and N=11 for warm acclimated trout). Different superscript letters indicate values that differ between acclimation states (ANOVA and a posteriori test, P≤0.05).

 

Figure 2
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  		Fig. 2. Time course of changes in maximal rates of pyruvate oxidation (nmol O min-1) by mitochondria from oxidative muscle during warm acclimation. State 3 rates are expressed as mg-1 mitochondrial protein, nmol-1 cytochrome and nmol-1 ANT. Values are means ± s.e.m. (N=11 for cold acclimated trout, N=8 for short-term warm exposed trout and N=11 for warm acclimated trout). Different superscript letters indicate rates at a given assay temperature that differ between acclimation states (ANOVA and a posteriori test, P≤0.05).

 

Figure 3
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  		Fig. 3. Time course of changes in cytochrome c oxidase activity during warm acclimation, expressed in UCCO mg-1 mitochondrial protein and UCCO nmol-1 cytochrome a (UCCO=µmol cytochrome c reduced min-1). Values are means ± s.e.m. (N=8 for cold acclimated trout, N=7 for short-term warm exposed trout and N=12 for warm acclimated trout). Different superscript letters indicate rates at a given assay temperature that differ between acclimation states (ANOVA and a posteriori test, P≤0.05).

 

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  		Fig. 4. Time course of changes in proportion of phospholipid classes and subclasses during warm acclimation. Values are means ± s.e.m. (N=4 for each acclimation state). Different superscript letters indicate values for a class or subclass that differ between acclimation states (ANOVA and a posteriori test, P≤0.05). Phospholipids are expressed as mol% of total moles of glycerophospholipids, calculated as indicated in Materials and methods. Abbreviations are defined in List of abbreviations.

 

Figure 5
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  		Fig. 5. Correlations between the activity of CCO and the main FA associated to CL of red muscle mitochondrial preparations. Activities plotted were measured at 5°C (squares) and 15°C (triangles). Lines represent statistically significant relationship.

 




© The Company of Biologists Ltd 2007