First published online April 18, 2006
Journal of Experimental Biology 209, 1737-1745 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02197
Rapid increase in the partial pressure of NH3 on the cutaneous surface of air-exposed mangrove killifish, Rivulus marmoratus
S. L. Litwiller1,
M. J. O'Donnell2 and
P. A. Wright1,*
1 Department of Integrative Biology, University of Guelph, Guelph, Ontario
N1G 2W1, Canada
2 Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1,
Canada

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Fig. 1. NH4+ concentration (A) and pH (B) of the bulk water
(black bars) and cutaneous surface locations on the body of the fish
(anterior, white bars; mid-section, hatched bars; posterior, cross-hatched
bars) in R. marmoratus immersed for 1 h in Series I experiments
(control I). Values are means + s.e.m. (N=8). Asterisk indicates
significant difference from bulk water (P<0.05). There were no
significant differences between locations.
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Fig. 2. NH4+ concentration (A) and pH (B) of the bulk water
(black bars) and cutaneous surface locations on the body of the fish
(anterior, white bars; mid-section, hatched bars; posterior, cross-hatched
bars) in R. marmoratus immersed for 11 days in Series I experiments
(control II). Values are means + s.e.m. (N=8). Asterisk indicates
significant difference from bulk water (P<0.05). There were no
significant differences between locations.
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Fig. 3. NH4+ concentration (A), pH (B) and the calculated
partial pressure of NH3 (C) of the bulk water (black bars) and
cutaneous surface of immersed (white bars) and acutely (1 h) air-exposed
(hatched bars) R. marmoratus (Series II). Values are means + s.e.m.
(N=5 bulk water, N=5 cutaneous immersed, N=9
cutaneous air-exposed). Bars with a different letter are significantly
different (P<0.05).
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Fig. 4. NH4+ concentration (A), pH (B) and the calculated
partial pressure of NH3 (C) of the bulk water (black bars) and
cutaneous surface of immersed (white bars) and chronically (11 days)
air-exposed (hatched bars) R. marmoratus (Series III). Values are
means + s.e.m. (N=8 bulk water, N=8 cutaneous immersed;
N=6 cutaneous air-exposed NH4+ concentration,
N=12 cutaneous air-exposed pH, N=6 cutaneous air-exposed
PNH3). Bars with a different letter are
significantly different (P<0.05).
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Fig. 5. Percent body water content over 11 days in both immersed (black bars) and
air-exposed (white bars) R. marmoratus. Values are expressed as mean
+ s.e.m. (N=6 immersed, N=8 air-exposed). Asterisk indicates
significant difference from day 0.
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© The Company of Biologists Ltd 2006