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First published online March 30, 2006
Journal of Experimental Biology 209, 1454-1462 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02141
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The postnatal development of neocortical neurons and glial cells in the Göttingen minipig and the domestic pig brain

Jacob Jelsing1,*, Rune Nielsen1, Aage Kristian Olsen2, Nanna Grand3, Ralf Hemmingsen4 and Bente Pakkenberg1

1 Research Laboratory for Stereology and Neuroscience, Copenhagen University Hospital, Bispebjerg, Denmark
2 PET Centre, Aarhus University Hospitals, Aarhus, Denmark
3 Ellegaard Göttingen Minipigs ApS, Slagelse, Denmark
4 Department of Psychiatry, Copenhagen University Hospital, Bispebjerg, Denmark


Figure 1
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  		Fig. 1. The optical fractionator sampling scheme. The cerebral hemispheres were divided medially (A), and the left or right hemisphere was selected systematically randomly before dehydration and infiltration in paraffin (B). The hemispheres were sectioned exhaustively into 40 µm-thick coronal sections (C), from which a predetermined fraction, the section sampling fraction (ssf), was sampled systematically randomly (D) and subsequently stained with Giemsa. Optical disectors were positioned systematically randomly on each of the sampled sections at regular predetermined x,y-positions (E,F). The area of the counting frame of the disector to the area associated with each x,y-step represents the area sampling fraction (asf). Counts were performed in all optical disectors hitting the structure of interest (E,F). Cellular nuclei were counted by moving the counting frame through a continuous stack of thin optical planes inside the section (G). The height of the disector (hdis) relative to the height of the section (t) represents the height sampling fraction (hsf). The disector is protected by upper and lower guard zones.

 

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  		Fig. 2. The postnatal development of neocortical cell numbers in the Göttingen minipig and the domestic pig brain. Total neuron number (A) and total glial cell number (B) in the Göttingen minipig. Total neuron number in the domestic pig (C).

 

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