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First published online March 30, 2006
Journal of Experimental Biology 209, 1395-1403 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02148

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Sweet problems: insect traits defining the limits to dietary sugar utilisation by the pea aphid, Acyrthosiphon pisum

A. E. Douglas^1,*, D. R. G. Price¹, L. B. Minto¹, E. Jones¹, K. V. Pescod¹, C. L. M. J. François¹, J. Pritchard² and N. Boonham³

¹ Department of Biology (Area 2), University of York, PO Box 373, York, YO10 5YW, UK
² Department of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
³ Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK

View larger version (20K): [in a new window]   Fig. 1. Aphid performance on diets of different sucrose concentration. (A) Lifetime survivorship. (B) Cumulative reproductive output per live aphid. (C) Relative growth rate of 6- to 8-day-old final instar larvae that had been raised from day 2 to day 6 on a diet with 0.5 mol l–1 sucrose (means ± s.e.m.). Values that are not significantly different (P>0.05) are indicated by the same letter.

View larger version (16K): [in a new window]   Fig. 2. Diet ingestion and assimilation by 6- to 8-day-old pea aphid clone LL01 on diets of different sucrose concentration. (A) Volume of diet ingested (means ± s.e.m.). (B) Dietary sucrose-carbon ingested (closed symbols) and assimilated (open symbols) (means ± s.e.m.). (C) Assimilation efficiency [100x(moles assimilated/moles ingested]. Values that are not significantly different (P>0.05) are indicated by the same letter.

View larger version (14K): [in a new window]   Fig. 3. Regression of aphid relative growth rate on volume of diet ingested on the 0.125 mol l–1 sucrose diet.

View larger version (15K): [in a new window]   Fig. 4. Osmotic pressure of the haemolymph of 8-day-old aphids reared on either chemically defined diets of different sucrose concentration (circles) or on plants (triangles) with phloem sap sucrose concentration as shown in Table 1. Values are means ± s.e.m. Mean values that are not significantly different (P>0.05) are indicated by the same letter.

View larger version (17K): [in a new window]   Fig. 5. Abundance and function of symbiotic bacteria Buchnera sp. in 8-day-old aphids on diets of different sucrose concentration. (A) Number of copies of Buchnera gene dnaK normalised to total DNA content. (B) Free amino acid content normalised to aphid mass. Values are means ± s.e.m. Mean values that are not significantly different (P>0.05) are indicated by the same letter.

View larger version (16K): [in a new window]   Fig. 6. Sucrase activity of 8-day-old aphids reared on diets of different sucrose concentration. Values are means ± s.e.m. Mean values that are not significantly different (P>0.05) are indicated by the same letter.

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