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First published online March 17, 2006
Journal of Experimental Biology 209, 1274-1284 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02093
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Effects of acclimation temperature and cadmium exposure on cellular energy budgets in the marine mollusk Crassostrea virginica: linking cellular and mitochondrial responses

Anton S. Cherkasov1, Pradip K. Biswas1, Daisy M. Ridings2, Amy H. Ringwood1 and Inna M. Sokolova1,*

1 Biology Department, University of North Carolina at Charlotte, 9201 University City Blvd, Charlotte, NC 28223, USA
2 Carolinas Medical Center, Cannon Research Center, 1542 Garden Terrace, Charlotte, NC 28203, USA


Figure 1
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  		Fig. 1. Effects of acclimation temperature on Cd accumulation (A) and oxygen consumption (B) in isolated gill and hepatopancreas cells of C. virginica. Oysters were exposed for 20 or 40 days to clean artificial seawater (ASW) or 50 µg l–1 Cd in ASW. Cd levels in tissues and isolated cells of control oysters were below the detection limits of the method used in this study (5 ng Cd g–1 dry tissue mass). N=6–12 in each group. Total oxygen consumption was measured in isolated cells of control oysters at their respective acclimation temperatures. N=6–12 in each group.

 

Figure 2
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  		Fig. 2. Effects of acclimation temperature (12, 20 and 28°C) and cadmium exposure on mitochondrial oxygen consumption rate (MO2) (A–C) and MO2 related to ATP turnover (D–F) in isolated gill and hepatopancreas cells of C. virginica. Data for control oysters and for those exposed for 20 or 40 days to 50 µg l–1 Cd are shown (N=6–12). Asterisks mark values that are significantly different from the respective controls (P<0.05).

 

Figure 3
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  		Fig. 3. Effects of acclimation temperature (12, 20 and 28°C) and cadmium exposure on mitochondrial MO2 related to protein synthesis (A–C) and proton leak (D–F) in isolated gill and hepatopancreas cells of C. virginica. Data for control oysters and for those exposed for 20 or 40 days to 50 µg l–1 Cd are shown (N=6–12). Asterisks mark values that are significantly different from the respective controls (P<0.05).

 

Figure 4
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  		Fig. 4. Effects of acclimation temperature and cadmium exposure on cellular energy budgets of isolated gill (A) and hepatopancreas (B) cells of C. virginica. Data for control oysters and for those exposed for 40 days to 50 µg l–1 Cd are shown (N=6–12). Proportions of the total oxygen consumption in isolated cells due to non-mitochondrial respiration (non-mito), mitochondrial proton leak (proton leak), cytosolic protein synthesis (protein synthesis) and the remaining ATP turnover (MATP) are given.

 




© The Company of Biologists Ltd 2006