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First published online February 15, 2006
Journal of Experimental Biology 209, 871-880 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02071

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Fuel use during glycogenesis in rainbow trout (Oncorhynchus mykiss Walbaum) white muscle studied in vitro

Jennifer C. Kam and C. Louise Milligan^*

Department of Biology, The University of Western Ontario, London, Ontario, Canada N6A 5B7

View larger version (31K): [in a new window]   Fig. 1. Glycogenic and oxidative fates of extracellular lactate during glycogenesis in muscle slices from exercised fish. (A) Muscle glycogen levels in tissue sampled immediately after exercise (Time 0) and in tissue incubated for 60 min in the substrates indicated. (B,C) The incorporation of 14C from lactate into the muscle glycogen (B) and CO2 produced (C). All values are given in nmol g–1 wet mass to facilitate comparisons between components. Data are presented as the mean ± 1 s.e.m. for slices from eight different fish (N=8). Columns within the same panel with the same letter are not significantly different from one another.

View larger version (27K): [in a new window]   Fig. 2. Glycogenic and oxidative fates of extracellular glycerol during glycogenesis in muscle slices from exercised fish. (A) Muscle glycogen levels in tissue sampled immediately after exercise (Time 0) and in tissue incubated for 60 min in the substrates indicated. (B,C) The incorporation of 14C from glycerol into the muscle glycogen (B) and CO2 produced (C). All values are given in nmol g–1 wet mass to facilitate comparisons between components. Data are presented as the mean ± 1 s.e.m. for slices from eight different fish (N=8). Columns within the same panel with the same letter are not significantly different from one another.

View larger version (29K): [in a new window]   Fig. 3. Glycogen synthesis and palmitate oxidation during glycogenesis in muscle slices from exercised fish. (A) Muscle glycogen levels in tissue sampled immediately after exercise (Time 0) and in tissue incubated for 60 min in the substrates indicated. (B) The incorporation of 14C from palmitate into CO2 produced. All values are given in nmol g wet mass–1 to facilitate comparisons between components. Data are presented as the mean ± 1 s.e.m. for slices from eight different fish (N=8). Columns within the same panel with the same letter are not significantly different from one another.

View larger version (27K): [in a new window]   Fig. 4. Muscle glycogen. (A) Glycogen levels in muscle slices obtained from fish immediately after exercise (Time 0), or after 60 min incubation in saline containing various substrates in equal concentration. (B,C) Incorporation of radiolabel from the various substrates into muscle glycogen (B) and CO2 produced (C). All values are given in nmol g–1 wet mass to facilitate comparisons between components. Data are presented as the mean ± 1 s.e.m. for slices from eight different fish (N=8). Columns within the same panel with the same letter are not significantly different from one another.

View larger version (21K): [in a new window]   Fig. 5. A proposed model describing extracellular substrate use in support of glycogenesis in trout muscle during recovery from exhaustive exercise. Thicker arrows indicate preferred pathways; wavy arrows indicate diffusion. LAe, lactate of extracellular origin; LAi, lactate of intracellular, glycolytic, origin; MCT, monocarboxylate transporter; PYR, pyruvate; LDH, lactate dehydrogenase; PDH, pryruvate dehydrogenase; GLY, glycerol; GK, glycerol kinase; G3P, glyerol 3-phosophate; DHAP, dihydroxyacetone phosphate; PA, palmitate, TCA, tricarboxylic acid cycle; CoA, coenzyme A; Ac-CoA, acetyl CoA; NAD+, oxidized nicotinamide-adenine dinucleotide; NADH, reduced nicotinamide-adenine dinucleotide; FAD, flavin adenine dinucleotide; FADH2, reduced FAD. See text for details (#, reaction number).

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