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First published online January 19, 2006
Journal of Experimental Biology 209, 510-517 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.01973
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Corticotropin-releasing hormone-receptor 1 (CRH-R1) and CRH-binding protein (CRH-BP) are expressed in the gills and skin of common carp Cyprinus carpio L. and respond to acute stress and infection

A. F. Mazon1, B. M. L. Verburg-van Kemenade1,*, G. Flik2 and M. O. Huising1,2

1 Cell Biology and Immunology, Department of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands
2 Department of Animal Physiology, Institute for Neuroscience, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands


Figure 1
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  		Fig. 1. Basal expression of CRH, CRH-R1 and CRH-BP in the gills and skin of C. carpio. Expression was assessed by RQ-PCR and is plotted relative to the expression of ribosomal 40S. Values are means ± s.d. (N=5).

 

Figure 2
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  		Fig. 2. Immunohistochemical demonstration of (A) CRH-positive and (B) CRH-BP-positive cells in the gills from C. carpio. Arrows indicate the immunoreactive cells. f, filament; sl, secondary lamellae; cvs, central venous sinus.

 

Figure 3
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  		Fig. 3. Immunohistochemical demonstration of (A,B) CRH-positive and (C,D) CRH-BP-positive cells in the skin from C. carpio. Arrows indicate the immunoreactive cells. e, epidermis; d, dermis.

 

Figure 4
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  		Fig. 4. (A) CRH immunoreactive cells and (B) CRH-BP immunoreactive cells in the phagocytic fraction from C. carpio gills. Arrows indicate the macrophage-like immunoreactive cells. (C) Expression of CRH, CRH-R1 and CRH-BP in the phagocytic fraction from carp gills. Expression was assessed by RQ-PCR and is plotted relative to the expression of ribosomal 40S. Values are means ± s.d. (N=4).

 

Figure 5
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  		Fig. 5. CRH-R1 and CRH-BP expression levels in (A) gills and (B) skin from C. carpio, 3 weeks after infection with T. borreli. Values are means ± s.d. (N=5). Asterisks indicate significant differences from the controls (P<0.05). Expression is standardised for ribosomal 40S expression and the relative quantification value is expressed as 2-{Delta}{Delta}Ct.

 

Figure 6
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  		Fig. 6. (A) CRH-R1 and CRH-BP expression levels in gills, (B) the total number of CRH-BP immunopositive cells and (C) CRH-R1 and CRH-BP expression in skin of C. carpio following 24 h restraint. Values are means ± s.d. (N=5). Asterisks indicate significant differences from the controls (P<0.05). Expression is standardised for ribosomal 40S expression and the relative quantification value is expressed as 2-{Delta}{Delta}Ct.

 




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