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First published online December 1, 2006
Journal of Experimental Biology 209, 4966-4973 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02595

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Beta3-Adrenoceptor in the eel (Anguilla anguilla) heart: negative inotropy and NO-cGMP-dependent mechanism

S. Imbrogno^1,2, T. Angelone^1,2, C. Adamo², E. Pulerà², B. Tota^2,* and M. C. Cerra^1,2

¹ Departments of Pharmaco-Biology, University of Calabria, 87030, Arcavacata di Rende, CS, Italy
² Departments of Cell Biology, University of Calabria, 87030, Arcavacata di Rende, CS, Italy

View larger version (9K): [in this window] [in a new window]   Fig. 1. Cumulative dose-response curve for BRL37344 on stroke volume (VS) and stroke work (WS) in isolated and perfused paced eel hearts. Percentage changes were evaluated as means ± s.e.m. (N=9). Significance of differences from control values (one-way ANOVA test) **P<0.01.

View larger version (6K): [in this window] [in a new window]   Fig. 2. Effects of BRL37344 (10 nmol l-1) before and after treatment with nadolol (10 µmol l-1) on stroke volume (VS) and stroke work (WS) in isolated and perfused paced eel hearts. Percentage changes were evaluated as means ± s.e.m. (N=6). One-way ANOVA was used for comparisons within groups; significance of differences from control values *P<0.05. Comparison between groups (two-way ANOVA, Duncan's test); P<0.05.

View larger version (8K): [in this window] [in a new window]   Fig. 3. The sigmoid concentration-response curves of BRL37344-mediated inhibition on VS of BRL37344 alone (0.1-100 nmol l-1) and of BRL 37344 plus a single concentration of SR59230 (10 nmol l-1) on the isolated and perfused working eel heart preparation. Inhibition of contractility is expressed as a percentage of VS [baseline=0%, peak inhibition by BRL37344 and BRL37344 plus SR59230= -100%]. The EC50 values (in log mol l-1) of BRL37344 alone was -7.68±0,12 (r2=0.98), of BRL 37344 plus SR59230 (10 nmol l-1) was -6.6±0.55 (r2=0.96). Comparison between groups (two-way ANOVA, Duncan's test); P<0.05 (N=8).

View larger version (5K): [in this window] [in a new window]   Fig. 4. Effects of isoproterenol (ISO; 100 nmol l-1) before and after treatment with SR59230 (10 nmol l-1) on stroke volume (VS) and stroke work (WS) in isolated and perfused paced eel hearts. Percentage changes were evaluated as means ± s.e.m. (N=7). One-way ANOVA was used for comparisons within groups; significance of differences from control values *P<0.05. Comparison between groups (two-way ANOVA, Duncan's test) P<0.05.

View larger version (5K): [in this window] [in a new window]   Fig. 5. Effects of BRL37344 (10 nmol l-1) before and after treatment with pertussis toxin (PTx; 0.01 nmol l-1) on stroke volume (VS) and stroke work (WS) in isolated and perfused paced eel hearts. Percentage changes were evaluated as means ± s.e.m. (N=6). One-way ANOVA was used for comparisons within groups; significance of differences from control values *P<0.05. Comparison between groups (two-way ANOVA, Duncan's test); P<0.05.

View larger version (7K): [in this window] [in a new window]   Fig. 6. Effects of BRL37344 (10 nmol l-1) before and after treatment with L-N5-(1-iminoethyl)ornitine (L-NIO; 10 µmol l-1), haemoglobin (Hb; 1 µmol l-1), 1H-(1,2,4)oxadiazolo-(4,3-a)quinoxalin-1-one (ODQ; 10 µmol l-1) and KT5823 (100 nmol l-1) on stroke volume (VS) and stroke work (WS) in isolated and perfused paced eel hearts. Percentage changes were evaluated as means ± s.e.m. (N=7-8 experiments for each drug). One-way ANOVA was used for comparisons within groups; significance of differences from control values *P<0.05. Comparison between groups (two-way ANOVA, Duncan's test) P<0.05.