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First published online October 18, 2006
Journal of Experimental Biology 209, 4319-4328 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02501
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The catch state of mollusc catch muscle is established during activation: experiments on skinned fibre preparations of the anterior byssus retractor muscle of Mytilus edulis L. using the myosin inhibitors orthovanadate and blebbistatin

Oleg Andruchov, Olena Andruchova and Stefan Galler*

Department of Cell Biology, University of Salzburg, Hellbrunnerstrasse 34, A-5020 Salzburg, Austria


Figure 1
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  		Fig. 1. Activation of skinned fibre bundles of anterior byssus retractor muscle (ABRM) with stepwise increase in Ca2+ concentrations. (A) Original recording of force and stiffness. The force spikes are induced by repetitive rectangular stretches of 20 ms duration and 0.1% L0 in amplitude. The free Ca2+ concentration is expressed as pCa (= -log[Ca2+]). The force recordings (insets) were obtained on another preparation; they show the force transients induced by rectangular stretches of about 5 s in duration and 0.2% L0 in amplitude applied at different stages of the experiment. (B) Relationship between stiffness and force at different Ca2+ activations in the experiment shown in A. The data points were obtained at the end of each individual Ca2+ activation.

 

Figure 2
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  		Fig. 2. Depression of force and stiffness of a maximally Ca2+-activated skinned anterior byssus retractor muscle (ABRM) fibre bundle by 10 mmol l-1 orthovanadate (Vi) and subsequent further depression by moderate alkalization. (A) Original recording of force and stiffness during changes of solutions as indicated. The force spikes are induced by repetitive rectangular stretches of 20 ms in duration and 0.1% L0 in amplitude. The broken line shows an experiment at pH 7.4. The insets show experiments on another ABRM fibre bundle; here force transients were induced by rectangular length changes of 0.2% L0 at different stages of the experiment. (B) Diagram showing the stiffness/force relationship of the experiment of Fig. 2A. Grey squares, Ca2+ activation; open circles, Vi; solid circles, moderate alkalization (change from pH 6.7 to pH 7.4) in the presence of Vi. The arrows indicate the time course of the experiment. (C,D) Data from similar experiments carried out at pH 7.4 (C) or in the presence of 100 µmol l-1 cAMP and 2 µmol l-1 cyclosporine A (CsA; D). Grey squares, Ca2+ activation; open circles, Vi application.

 

Figure 3
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  		Fig. 3. Depression of force and stiffness of a maximally Ca2+-activated skinned anterior byssus retractor muscle (ABRM) fibre bundle by 5 µmol l-1 blebbistatin. (A) Original recording of force and stiffness during changes of solutions as indicated. The force spikes are induced by repetitive rectangular stretches of 20 ms in duration and 0.1% L0 in amplitude. The broken line shows an experiment at pH 7.4. The insets show experiments on another ABRM fibre bundle; here force transients were induced by rectangular length changes of 0.2% L0 at different stages of the experiment. (B) Diagram showing the stiffness/force relationship of the experiment shown in A. Grey squares, Ca2+ activation; open circles, blebbistatin. (C,D) Data from similar experiments carried out at pH 7.4 (C) or in the presence of 100 µmol l-1 cAMP and 2 µmol l-1 cyclosporine A (CsA). Grey squares, Ca2+ activation; open circles, blebbistatin application.

 

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© The Company of Biologists Ltd 2006