QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online January 3, 2006
Journal of Experimental Biology 209, 372-379 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02006

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chauvigné, F. Articles by Rescan, P. Y. Search for Related Content PubMed PubMed Citation Articles by Chauvigné, F. Articles by Rescan, P. Y.

In situ hybridisation of a large repertoire of muscle-specific transcripts in fish larvae: the new superficial slow-twitch fibres exhibit characteristics of fast-twitch differentiation

F. Chauvigné, C. Ralliere, C. Cauty and P. Y. Rescan^*

National Institute for Agricultural Research, the Joint Unit Research for Fish Physiology, Biodiversity and the Environment, INRA Scribe, IFR140, Campus de Beaulieu, 35042 Rennes, France

View larger version (98K): [in a new window]   Fig. 1. Expression of fast myosin heavy chain (MyHC; A,D,G), fast myosin binding protein C (B,E,H), and fast tropomyosin (C,F,I) in trout larvae. Transverse sections through the myotome at 27 (A–C), 41 (D–F) and 50 (G–I) days post-fertilisation. Fast isoform transcripts initially present throughout the whole fast muscle mass (A–C) become confined to the edges of adjacent myotomes (D–I). Scale bars, 200 µm.

View larger version (100K): [in a new window]   Fig. 2. Merged immunofluorescence images of transverse sections of fast muscle at 48 days post-fertilisation. Expression of (A) fast myosin heavy chain (MyHC; green) and fast troponin C (red), (B) fast MyHC (green) and fast tropomyosin (red), (C) fast MyHC (green) and fast myosin light chain 1 (MyLC1; red). Incomplete overlap of the fluorescent markers (arrows) show that fast muscle fibres contain differing proportions of fast-twitch muscle isoform transcripts. Scale bars, 100 µm.

View larger version (58K): [in a new window]   Fig. 3. Expression of slow myosin light chain 1 (MyLC1; red) and cytochrome C oxidase subunit IV (green) transcripts in 48 days post-fertilisation trout larvae. Separate (A,B) and merged (C) immunofluorescence images of transverse sections at the horizontal septum. Cytochrome c oxidase subunit IV transcripts accumulate in slow MyLC1-expressing superficial muscle fibres (arrows) and in lateral fast muscle fibres (arrowheads). Scale bars, 80 µm.

View larger version (171K): [in a new window]   Fig. 4. Transverse section of lateral muscle at 44 days post-fertilisation. Semi-thin section showing region around the horizontal septum; Richardson stain. New small diameter slow fibres (nSF) are added laterally to the original embryonic monolayer of slow fibres (eSF). FF, fast fibres; ep, epidermis. Scale bars, 20 µm.

View larger version (140K): [in a new window]   Fig. 5. Expression of slow myosin light chain 1 (MyLC1; A,B) and slow troponin T (C,D). Transverse sections at the horizontal septum of trout larvae 41 (A,C) and 76 (B,D) days post-fertilisation. Slow MyLC1 and slow troponin T isoform transcripts accumulate in all the slow muscle fibres. Scale bars, 40 µm.

View larger version (166K): [in a new window]   Fig. 6. Expression of fast myosin heavy chain (MyHC; A,E), fast myosin binding protein C (B,F), fast myosin light chain 1 (MyLC1; C,G), fast MyLC3 (D,H), and slow MyLC1 (green) and fast MyHC (red) (I–K) in trout larvae. Transverse sections of lateral myotomal muscle at 41 (A–D and I), 48 (J) and 76 (E–H,K) days post-fertilisation. Labelling for fast muscle isoforms is observed in fast muscle fibres and in the superficial layers of slow fibres (arrows, A–H). Merged immunofluorescence images (I–K) show that the most external layers of superficial fibres express both slow and fast muscle isoforms (see arrows showing the overlap of the two fluorescent markers). Scale bars, 40 µm (A–D); 80 µm (E–H); 40 µm (I–J) and 80 µm (K).