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First published online September 19, 2006
Journal of Experimental Biology 209, 3806-3811 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02424
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The expression level of frog relaxin mRNA (fRLX), in the testis of Rana esculenta, is influenced by testosterone

Gianluca De Rienzo1, Francesco Aniello2, Margherita Branno3, Gaia Izzo1 and Sergio Minucci1,*

1 Dipartimento di Medicina Sperimentale, Sez. Fisiologia Umana e Funzioni Biologiche Integrate `F. Bottazzi', Seconda Università di Napoli, via Costantinopoli 16, 80138 Napoli, Italy
2 Dipartimento di Biologia Strutturale e Funzionale, Università di Napoli `Federico II', via Cinthia, 80126 Napoli, Italy
3 Laboratorio di Biochimica e Biologia Molecolare, Stazione Zoologica `A. Dohrn', Villa Comunale, 80121 Napoli, Italy


Figure 1
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  		Fig. 1. Agarose gel electrophoresis of RT-PCR products. (A) fRLX mRNA expression on frog testis treated with T and T in combination with CPA at 2, 8 and 24 h. (B) fP1 expression on the same samples used as control. Control, initial control frog testis. Lane C, control PCR without cDNA; M, molecular mass marker VIX (Roche Diagnostic). (C) Relative density of the fRLX and fP1 band measured with Gel Doc.

 

Figure 2
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  		Fig. 2. (A) Northern blot analysis of total RNA extracted from frog testis hybridized with the fRLX cDNA as probe. Each lane contained 20 µg of total RNAs extracted from testis incubated with vehicle alone (Krebs), testosterone (T) and T in combination with CPA. (B) The same filter was hybridized with the fP1 cDNA probe as control. (C) Relative density of the fRLX and fP1 band measured with Gel Doc.

 

Figure 3
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  		Fig. 3. In vivo CPA treatment. (A) Levels of fRLX mRNA determined by RT-PCR on initial control (lane 3), 15 days control (lane 4) and 15 days CPA-treated (lane 5) frog testis. Lane 1: molecular mass marker VIX (Roche Diagnostic); lane 2: control PCR. (B) fP1 mRNA levels used as control. (C,D) In situ hybridization of fRLX antisense mRNA on the control (C) and in the CPA-treated frog testis (D).

 




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