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First published online June 15, 2006
Journal of Experimental Biology 209, 2567-2575 (2006)
Published by The Company of Biologists 2006
doi: 10.1242/jeb.02270

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2-hydroxyestradiol-17ß-induced oocyte maturation: involvement of cAMP–protein kinase A and okadaic acid-sensitive protein phosphatases, and their interplay in oocyte maturation in the catfish Heteropneustes fossilis

A. Mishra and K. P. Joy^*

Department of Zoology, Banaras Hindu University, Varanasi-221005, India

View larger version (28K): [in a new window]   Fig. 1. Effects of 2-hydroxyestradiol-17ß (2-OHE2, 5 µmol l–1) on germinal vesicle breakdown (GVBD, line curves) and cAMP level (bars) in Heteropneustes fossilis. Values are means ± s.e.m. (N=3 for GVBD assay; N=5 for cAMP assay). Data were analyzed by one-way ANOVA (P<0.001) and Newman–Keuls' test (P<0.05). Asterisks indicate significant difference from the respective vehicle control groups (P<0.05).

View larger version (34K): [in a new window]   Fig. 2. In vitro effects of incubation of postvitellogenic follicles with 2-hydroxyestradiol-17ß (2-OHE2, 5 µmol l–1) and different concentrations of cAMP, IBMX, theophylline or caffeine on the percentage of germinal vesicle breakdown (GVBD) in Heteropneustes fossilis. Values are means ± s.e.m. (N=3). Data were analyzed by one-way ANOVA (P<0.001) and Newman–Keuls' test (P<0.05). All the groups are significantly different from the vehicle control (VC) group. *Significant difference from the 2-OHE2 group (P<0.05).

View larger version (36K): [in a new window]   Fig. 3. (A,B) In vitro effects of different concentrations of H89 on the percentage germinal vesicle breakdown (GVBD) in Heteropneustes fossilis. (A) H89 alone or co-incubated with 2-hydroxyestradiol-17ß (2-OHE2, 5 µmol l–1) for 24 h. Values are mean ± s.e.m. (N=3). (B) The effect of H89 on IBMX inhibition of 2-OHE2-induced GVBD. Data were analyzed by one-way ANOVA (P<0.001) and Newman–Keuls' test (P<0.05). Asterisks indicate significant difference from the vehicle control (VC) group. Letters indicate comparisons with the 2-OHE2 (A) or H89 (B) group. Numbers show comparisons with 2-OHE2 (B) group. Groups marked with the same letter or number are not significantly different and that with different letters or numbers are significantly different. NS, not significant from the vehicle control (VC) group.

View larger version (28K): [in a new window]   Fig. 4. (A,B) In vitro effects of different concentrations of okadaic acid (OA) on the percentage of 2-OHE2-induced germinal vesicle breakdown (GVBD) in Heteropneustes fossilis. (A) Effect of OA alone or co-incubated with 2-hydroxyestradiol-17ß (2-OHE2, 5 µmol l–1) for 24 h. (B) The effects of OA on IBMX inhibition of 2-OHE2-induced GVBD. Values are mean ± s.e.m. Data were analyzed by one-way ANOVA (P<0.001) and Newman–Keuls' test (P<0.05). Asterisks indicate significant difference from the vehicle control (VC) group. Letters indicate comparisons with the 2-OHE2 (A) or OA (B) group. Numbers show comparisons with 2-OHE2 (B) group. Groups marked with the same letter or number are not significantly different and those with different letters or numbers are significantly different. NS, not significant from the vehicle control (VC) group.

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