First published online March 21, 2005
Journal of Experimental Biology 208, 1267-1276 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01523
Statistical analysis of modified complete randomized designs: applications to chemo-orientation studies
Ramani S. Pilla1,*,
David J. Kitska2 and
Catherine Loader2
1 Department of Statistics and Department of Biology, Case Western Reserve
University, Cleveland, OH 44106, USA
2 Department of Statistics, Case Western Reserve University, Cleveland, OH
44106, USA

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Fig. 1. A design layout under a type of restricted randomization for three blocks,
in which multiple subjects were challenged to a single treatment before
administering the next treatment. The order of the treatments as well as the
assignment of the subjects within each block were randomized.
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Fig. 2. An illustration of P. americana males tracking female pheromone
upwind (right to left) in a laboratory wind tunnel, containing the
time-averaged plume boundaries of titanium tetrachloride smoke plume in 25 cm
s1 wind. Each circle represents the body position at every
0.083 s. (A) Point source plume, 2.4 cm wide at the source spreads to 7.7 cm
wide at the downwind end. (B) Ribbon plume, 1.5 cm wide at the source spreads
to 6.1 cm wide at the downwind end. (C) Wide plume, 17 cm wide at the source
spreads to 26.5 cm at the downwind end. (D) Cylinder plume, 7.6 cm wide at the
source spreads to 68.1 cm at the downwind end.
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Fig. 3. A completely randomized design layout for a single block in which every
subject and every treatment were individually randomized.
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Fig. 4. The response variable ground speed (cm s1) for the P.
americana experiment sorted first by day and next by pheromone.
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Fig. 5. The response variable ground speed (cm s1) for the P.
americana experiment sorted first by pheromone (Pher) and then by
day.
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© The Company of Biologists Ltd 2005