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First published online March 14, 2005
Journal of Experimental Biology 208, 1201-1208 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01492
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Physiological and morphological correlates of among-individual variation in standard metabolic rate in the leopard frog Rana pipiens

Anthony C. Steyermark*, Alexander G. Miamen, Hesam S. Feghahati and Anthony W. Lewno

Department of Biology, University of St Thomas, St Paul, MN 55105, USA



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Fig. 1. Standard metabolic rate (SMR) of 26 leopard frogs increased with body mass (SMR=-0.558+0.060 xmass; r2=0.19; P=0.025).

 


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Fig. 2. Residual SMR increased with residual kidney mass (r2=0.25; P=0.01), but not with residuals of gastrocnemius, heart, liver or small intestine.

 


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Fig. 3. SMR increased with serum T4 thyroxine concentration (SMR=-4.84+8.023[thyroxine]; r2=0.16; P=0.04).

 


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Fig. 4. Residual SMR increased with liver residual relative mitochondrial content (r2=0.15; P=0.05). Relative mitochondrial content was determined by incubating disaggregated liver tissue with MitoTracker Deep Red 633 mitochondria stain, and measuring the fluorescence intensity of bound stain by flow cytometry. Fluorescence intensity is dimensionless.

 


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Fig. 5. Residual relative mitochondrial content increased with residual serum T4 (r2=0.17; P=0.03).

 

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© The Company of Biologists Ltd 2005