QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online March 14, 2005
Journal of Experimental Biology 208, 1045-1052 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01506

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fellner, S. K. Articles by Parker, L. Search for Related Content PubMed PubMed Citation Articles by Fellner, S. K. Articles by Parker, L.

Endothelin-1, superoxide and adeninediphosphate ribose cyclase in shark vascular smooth muscle

Susan K. Fellner^1,2,* and Laurel Parker¹

¹ Mount Desert Island Biological Laboratory, Salisbury Maine 04672 USA
² Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7545, USA

View larger version (14K): [in a new window]   Fig. 1. Representative tracing of cytosolic Ca2+ ([Ca2+]i) response of shark anterior mesenteric artery vascular smooth muscle (VSM) to endothelin-1 (ET-1) in Ca2+-free shark Ringers followed by the addition of Ca2+ (2.5 mmol l-1).

View larger version (19K): [in a new window]   Fig. 2. Effect of inhibitors of ADPR cyclase on endothelin-1 (ET-1)-stimulated increases in [Ca2+]i in vascular smooth muscle (VSM). (A) Representative tracing of the [Ca2+]i response to ET-1 in the presence and absence of nicotinamide. (B) Representative tracing of the response to ET-1 in the presence and absence of Zn2+. (C) Summary data of the effects of nicotinamide (N=15) and Zn2+ (N=11) on the ET-1-induced peak elevation of [Ca2+]i. *P<0.01 for both inhibitors vs ET-1 alone.

View larger version (18K): [in a new window]   Fig. 3. Effect of NAD(P)H oxidase inhibitor (DPI) and superoxide dismutase mimetic (TEMPOL) on endothelin-1 (ET-1) stimulation of [Ca2+]i in shark vascular smooth muscle (VSM). (A) Typical tracing of the [Ca2+]i response to ET-1 in the presence and absence of DPI. (B) Typical tracing of the ET-1 in the presence and absence of TEMPOL. (C) Summary data showing the inhibitory effects of DPI (N=19) and TEMPOL (N=19) on the ET-1 induced elevation of [Ca2+]i. *P<0.01 for ET-1 plus DPI or TEMPOL vs ET-1 alone.

View larger version (18K): [in a new window]   Fig. 4. Effects of combining inhibitors of the IP3 receptor with an inhibitor of NAD(P)H oxidase (DPI) or a superoxide scavenger (TEMPOL) in Ca2+-free shark Ringers. In each case, the combination of antagonists blocked the [Ca2+]i response to endothelin-1 (ET-1) by 83±1%. *P<0.01.