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First published online November 4, 2005
Journal of Experimental Biology 208, 4283-4290 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01899

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Thermally induced changes in lipid composition of raft and non-raft regions of hepatocyte plasma membranes of rainbow trout

John K. Zehmer^* and Jeffrey R. Hazel

School of Life Sciences, Arizona State University, Tempe, AZ, USA

View larger version (22K): [in a new window]   Fig. 1. Raft-enriched plasma membrane (raft) and raft-depleted plasma membrane (RDPM) phospholipid class composition. Total lipids extracted from isolated membranes were separated by TLC and the major phospholipid classes were quantified by phosphate assay. (A) RDPM from 5°C-acclimated (open bars) and 20°C-acclimated (filled bars) trout. (B) Raft from 5°C-acclimated (open bars) and 20°C-acclimated (filled bars) trout. (C) RDPM (open bars) and raft (filled bars) from 5°C-acclimated trout. (D) RDPM (open bars) and raft (filled bars) from 20°C-acclimated trout. PtdCho, phosphatidylcholine; PtdEtn, phosphatidylethanolamine; PtdIns, phosphatidylinositol; PtdSer, phosphatidylserine; SM, sphingomyelin. Values are means + S.E.M. (N=4).

View larger version (14K): [in a new window]   Fig. 2. Indexes of total fatty acid unsaturation of membrane fractions. (A) Percentage of unsaturated fatty acids and unsaturation index of raft (triangles) and RDPM (squares) from 5°C-acclimated (open symbols) and 20°C-acclimated (filled symbols) fish. The dotted lines are isopleths representing the relationship between percentage unsaturation and unsaturation index if each unsaturated fatty acid had three (N=3) or four (N=4) double bonds. (B) Percentage of monounsaturated fatty acids (MUFA) and (C) percentage of polyunsaturated fatty acids (PUFA) in raft and RDPM from 5°C-acclimated (open bars) and 20°C-acclimated (filled bars) fish. Bars with a common lower case letter do not differ significantly. All values are means ± S.E.M., N=4. Raft, raft-enriched plasma membrane; RDPM, raft-depleted plasma membrane.

View larger version (14K): [in a new window]   Fig. 3. Indexes of fatty acid unsaturation of phosphatidylcholine in membrane fractions. (A) Percentage of unsaturated fatty acids and unsaturation index of phosphatidylcholine of raft (triangles) and RDPM (squares) from 5°C-acclimated (open symbols) and 20°C-acclimated (filled symbols) fish. The dotted lines are isopleths, as described in Fig. 2. (B) Percentage of monounsaturated fatty acids (MUFA) and (C) percentage of polyunsaturated fatty acids (PUFA) in raft and RDPM from 5°C-acclimated (open bars) and 20°C-acclimated (filled bars) fish. Bars with a common lower case letter do not differ significantly. All values are means ± S.E.M., N=4. Raft, raft-enriched plasma membrane; RDPM, raft-depleted plasma membrane.

View larger version (15K): [in a new window]   Fig. 4. Indexes of fatty acid unsaturation of phosphatidylethanolamine in membrane fractions. (A) Percentage of unsaturated fatty acids and unsaturation index of phosphatidylethanolamine of raft (triangles) and RDPM (squares) from 5°C-acclimated (open symbols) and 20°C-acclimated (filled symbols) fish. The dotted lines are isopleths, as described in Fig. 2. (B) Percentage of monounsaturated fatty acids and (C) percentage of polyunsaturated fatty acids in raft and RDPM from 5°C-acclimated (open bars) and 20°C-acclimated (filled bars) fish. All values are means ± S.E.M., N=4. Raft, raft-enriched plasma membrane; RDPM, raft-depleted plasma membrane.