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First published online November 4, 2005
Journal of Experimental Biology 208, 4193-4198 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01862
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Muscle biochemistry and the ontogeny of flight capacity during behavioral development in the honey bee, Apis mellifera

Stephen P. Roberts* and Michelle M. Elekonich

Department of Biological Sciences, University of Nevada, Las Vegas, 4505 S. Maryland Parkway, Las Vegas, NV 89154-4004, USA



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  		Fig. 1. Hsp70 expression in honey bee nurses and foragers. (A,B) Hsp70 expression in heads; (C,D) Hsp70 expression in thoraces. Hsp70 family proteins were isolated on western blots labeled with a Hsp70 mouse monoclonal antibody (Sigma, St Louis, MO, USA; H5147) and an anti-mouse secondary antibody conjugated to horseradish peroxidase (Sigma). Proteins were visualized with enhanced chemiluminescence and quantified on a Typhoon Phosphorimager (Amersham, UK). The Hsp70 standard is 0.5 µg of purified Hsp70 protein (from bovine brain; Sigma; H9776). Numbers in bars indicate sample size.

 

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© The Company of Biologists Ltd 2005