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First published online October 7, 2005
Journal of Experimental Biology 208, 3971-3986 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01849

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An updated catalogue of salivary gland transcripts in the adult female mosquito, Anopheles gambiae

Bruno Arcà^1,2, Fabrizio Lombardo², Jesus G. Valenzuela³, Ivo M. B. Francischetti³, Osvaldo Marinotti⁴, Mario Coluzzi² and José M. C. Ribeiro^3,*

¹ Department of Structural and Functional Biology, University "Federico II", 80126 Naples, Italy
² Parasitology Section, Department of Public Health, University "La Sapienza", 00185 Rome, Italy
³ Medical Entomology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, Rockville, MD 20852, USA
⁴ Department of Molecular Biology and Biochemistry and Department of Biological Chemistry, University of California, Irvine, CA 92697, USA

View larger version (17K): [in a new window]   Fig. 1. Wolbachia-like genes of Anopheles gambiae in chromosome 3R. Blue boxes represent automatic annotation of putative genes. Dark grey boxes represent matching location of expressed sequence tags. Vertical dark lines represent stop codons on selected forward (top) or reverse (bottom) frame.

View larger version (18K): [in a new window]   Fig. 2. Transposable elements (TE5p and TE3p) flanking the 5' and 3' Wolbachia gene region in Anopheles gambiae chromosome 3R.

View larger version (45K): [in a new window]   Fig. 3. BLAST result analysis of transposable elements (TE5p and TE3p) flanking the Wolbachia gene region in chromosome 3R. (A) TE5p. (B) TE6p.

View larger version (12K): [in a new window]   Fig. 4. Diagram of the D7 gene cluster on chromosome arm 3R. The arrow representing Contig_709 indicates a non-coding RNA mapping to the 5/region of the D7 short cassette.

View larger version (8K): [in a new window]   Fig. 5. Diagram of the SG1 gene cluster on chromosome X of Anopheles gambiae. Blue bars represent genes, and dark bars represent RNA sequences found in the salivary transcriptome of adult females.

View larger version (78K): [in a new window]   Fig. 6. Clustal alignment of the gSG1/Trio family of salivary proteins. Cysteines are marked with black boxes; conserved residues are marked with blue boxes.

View larger version (44K): [in a new window]   Fig. 7. Tissue specificity of genes expressed in the salivary glands of adult female Anopheles gambiae mosquitoes as evidenced by RT–PCR analysis. Total RNA from female salivary glands (sg), carcasses (c; adult females with salivary glands removed) or adult males (m) was amplified using the gene-specific primers indicated on the right. Amplifications are grouped according to pattern of expression: (A) housekeeping; (B) female gland specific; (C) enriched in female glands; (D) female glands and adult males; (E) rpS7, ribosomal protein S7 used for normalization.

View larger version (26K): [in a new window]   Fig. 8. Tissue-specific alternative splicing of sal_tryp XII. Total RNA from sg, c and m (see legend to Fig. 7) was amplified by RT–PCR. The length in base pair of the amplified fragments is indicated.

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