First published online October 7, 2005
Journal of Experimental Biology 208, 3859-3871 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01853
Differential freshwater adaptation in juvenile sea-bass Dicentrarchus labrax: involvement of gills and urinary system
Catherine Nebel*,
Bernard Romestand,
Geneviève Nègre-Sadargues,
Evelyse Grousset,
Fabien Aujoulat,
Julien Bacal,
François Bonhomme and
Guy Charmantier
Laboratoire Génome, Populations, Interactions, Adaptation, UMR
5171, Université Montpellier II, Place E. Bataillon, 34095 Montpellier,
Cedex 05, France
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Fig. 1. Blood and urine osmolality in SWS, FWS and FWU Dicentrarchus
labrax (N=12–19). Different letters indicate significant
differences (P<0.001).
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Fig. 2. Gill sections of SWS (A,D), FWS (B,E) and FWU (C,F) Dicentrarchus
labrax. Chloride cells are specifically stained (black) with
Champy-Maillet's fixative (A–C) or immunostained (arrows) by
immunofluorescence to localize the Na+/K+-ATPase
(D–F). Fi, filament; La, lamella. Scale bars, 20 µm (A–C), 10
µm (D–F).
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Fig. 3. Number of gill chloride cells (including all filamentary and lamellar
ionocytes) in SWS, FWS and FWU Dicentrarchus labrax on a total length
of 10 interlamellar spaces (N=5–11). Observations from
Champy-Maillet-stained sections. Different letters indicate significant
differences (SWS/FWS, SWS/FWU: P<0.001; FWS/FWU:
P<0.01).
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Fig. 4. Ultrastructure of a gill filamentary chloride cell of FWU Dicentrarchus
labrax. Note the presence of numerous mitochondria in the median part of
the cell and the well-developed tubular system that is an extension of the
basolateral cell membrane (A,B). The apical cell part presents a few
microridges (A,C). BM, basement membrane; CC, chloride cell; J, junction; MI,
mitochondria; MR, microridges; PVC, pavement cell; TS, tubular system. Scale
bars, 2 µm (A), 0.7 µm (B), 1 µm (C).
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Fig. 5. Gill Na+/K+-ATPase abundance (A) and activity (B) in
SWS, FWS and FWU Dicentrarchus labrax. Different letters indicate
significant differences. (A) N=7; SWS/FWS: P<0.01,
SWS/FWU: P<0.001; (B) N=9; SWS/FWS: P<0.05,
FWS/FWU: P<0.01, SWS/FWU: P<0.001.
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Fig. 6. Longitudinal horizontal sections of Dicentrarchus labrax kidney in
SWS (A,B), FWS (C,D) and FWU (E,F). BS, blood sinus; CD, collecting duct; DA,
dorsal aorta; M, muscle; SB, swim bladder; UT, urinary tubule. Scale bars, 120
µm (A,C,E), 60 µm (B,D,F).
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Fig. 7. Percentage area of the kidney tubules compared to the total kidney area
(minus the ducts and the dorsal aorta) in SWS, FWS and FWU Dicentrarchus
labrax (N=4–5). Different letters indicate significant
differences (P<0.05).
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Fig. 8. Localization of the Na+/K+-ATPase through
immunofluorescence in the urinary system in SWS (A,B), FWS (C,D) and FWU (E,F)
Dicentrarchus labrax juveniles. Note the absence of immunostaining at
the dorsal part of the SWS urinary bladder (arrow). CD, collecting duct; UB,
urinary bladder; UT, urinary tubule. Scale bars, 100 µm (A,C,E), 20 µm
(B,D,F).
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Fig. 9. Renal Na+/K+-ATPase abundance (A) and activity (B) in
SWS, FWS and FWU Dicentrarchus labrax. Different letters indicate
significant differences. (A) N=48; SWS/FWS: P<0.01,
FWS/FWU: P<0.05; (B) N=9–14;
P<0.001.
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© The Company of Biologists Ltd 2005
