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First published online January 5, 2005
Journal of Experimental Biology 208, 309-316 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01410

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The influence of environmental P_O2 on hemoglobin oxygen saturation in developing zebrafish Danio rerio

Sandra Grillitsch^1,2, Nikolaus Medgyesy^1,2, Thorsten Schwerte^1,2 and Bernd Pelster^1,2,*

¹ Institute for Zoology and Limnology, University of Innsbruck, Austria
² Center for Molecular Biosciences, Innsbruck, Austria

View larger version (18K): [in a new window]   Fig. 1. Schematic drawing of the experimental chamber with an embedded zebrafish larva.

View larger version (12K): [in a new window]   Fig. 2. Maximum differences in light absorption of oxygenated and deoxygenated zebrafish blood between 2 d.p.f. and 12 d.p.f. Values are means ± S.E.M.; N values are given in parentheses above each symbol.

View larger version (18K): [in a new window]   Fig. 3. (A) Number of zebrafish larvae showing partial deoxygenation of the blood under normoxic conditions (filled bar) and the number of animals, showing complete oxygen saturation (open bar). (B) Oxygen saturation of the blood in the ventricle of normoxic zebrafish larvae (PO2=20 kPa). Values are means ± S.E.M. *Significant differences from full saturation. N values are given in parentheses below each symbol.

View larger version (14K): [in a new window]   Fig. 4. Oxygenation of blood in the ventricle under progressive hypoxia in 2 d.p.f. animals (N=12) and at 8 d.p.f. (N=8); *Significant differences from control values recorded at normoxia (PO2=20 kPa).

View larger version (19K): [in a new window]   Fig. 5. Heart rate of zebrafish larvae between 2 d.p.f. and 12 d.p.f. under normoxic (PO2=20 kPa) and hyperoxic (PO2=100 kPa) conditions. Values are means ± S.E.M.; *Significant differences between normoxia and hyperoxia (P<0.05). N values are given in parentheses below each symbol.

View larger version (17K): [in a new window]   Fig. 6. Heart rate of zebrafish larvae between 2 d.p.f. and 12 d.p.f. under normoxic conditions (PO2=20 kPa) and progressive hypoxia down to anoxic conditions. Values are means ± S.E.M. *Significant differences between normoxia and hypoxic conditions (P<0.05). N values are given in parentheses below each symbol.

View larger version (15K): [in a new window]   Fig. 7. Oxygen consumption of developing zebrafish larvae between 2 d.p.f. and 15 d.p.f., Values for animals between 1 and 9 d.p.f. were measured in a twin flow respirometer. Values are means ± S.E.M. from seven experiments. Within each experiment 10–15 eggs were pooled. Values for 11, 13 and 15 d.p.f. were taken from Bagatto et al. (2001), originally measured at 25°C. Data were recalculated to 28°C using a Q10 of 3.2, as determined for 10 d.p.f. animals for the temperatures between 25°C and 28°C (Barrionuevo and Burggren, 1999). *Significant differences relative to the previous day (P<0.05).

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