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First published online January 5, 2005
Journal of Experimental Biology 208, 267-275 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01372

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Electrical activity of caudal neurosecretory neurons in seawater and freshwater-adapted Platichthys flesus, in vivo

A. J. Ashworth, J. R. Banks, M. J. Brierley, R. J. Balment and C. R. McCrohan^*

Faculty of Life Sciences, The University of Manchester, Manchester M13 9PT, UK

View larger version (24K): [in a new window]   Fig. 1. Activity of T1 and T2 Dahlgren cells in SW- and FW-adapted flounder, recorded intracellularly. (A) Responses to depolarising current injection (1.4 nA, 500 ms) are similar in SW and FW-adapted fish. Note large amplitude AHP in T1 and characteristic spike frequency accommodation in T2. (B) Activity patterns in T1 cells (all SW-adapted): B, typical spontaneous bursting; P, phasic; T, tonic. (C) Single burst recorded from a Dahlgren cell with instantaneous spike frequency plot (top), showing how burst parameters were defined.

View larger version (29K): [in a new window]   Fig. 2. Extracellular multiunit recording from CNSS of SW-adapted flounder in vivo, showing raw trace (top) and activity of ten individual units separated by off-line Spike2 analysis. Dahlgren cells show a range of firing activity patterns, including: T, tonic; P, phasic; B, bursting.

View larger version (35K): [in a new window]   Fig. 3. Extracellular multiunit recording from CNSS of SW-adapted flounder in vivo, showing apparent correlation of activity in 11 different units (separated by Spike2 analysis), which combines to reveal periodic increases in overall activity in the raw trace (top).

View larger version (26K): [in a new window]   Fig. 4. Extracellular multiunit recording from CNSS of SW-adapted flounder. Cutting the spinal cord just above the recording site (arrow) led to increased activity including recruitment of previously silent units and enhanced bursting activity.

View larger version (32K): [in a new window]   Fig. 5. Extracellular multiunit recording from CNSS of SW-adapted flounder, showing response to stimulation of branchial branch of the vagal nerve (A, arrow), and mild pinch to lip (B) or fin (C). All three treatments led to an overall reduction in activity lasting around 400 s.