QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online January 5, 2005
Journal of Experimental Biology 208, 223-232 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01362

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JEB Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brown, J. A. Articles by Rankin, J. C. Search for Related Content PubMed PubMed Citation Articles by Brown, J. A. Articles by Rankin, J. C. Social Bookmarking                         What's this?

Activation of the newly discovered cyclostome renin–angiotensin system in the river lamprey Lampetra fluviatilis

J. Anne Brown^1,*, Christopher S. Cobb^1,, Susan C. Frankling¹ and J. Cliff Rankin^2,

¹ School of Biological and Chemical Sciences, Hatherly Laboratories, University of Exeter, Prince of Wales Road, Exeter, EX4 4PS, UK
² Aquatic Biology Research Centre, Institute of Biology, University of Southern Denmark – Odense University, Hindsholmvej 11, DK-5300 Kerteminde, Denmark

View larger version (15K): [in a new window]   Fig. 1. Parallelism of the [Asn1,Val5]-Ang II radioimmunoassay standard curve (shown as means ± S.E.M., N=6) and serial dilution of an extract of pooled plasma from the river lamprey Lampetra fluviatilis. For details of the assay, see Materials and methods.

View larger version (25K): [in a new window]   Fig. 2. Blood volume depletion in river lampreys acclimated to Kerteminde seawater at 576 mOsm kg–1 (21 p.p.t.). The first blood sample (pre-volume depletion; open bars) was taken to achieve a 40% decrease in blood volume and a second sample (post-volume depletion; hatched bars) was collected after 30 min (N=10), 60 min (N=11) or 90 min (N=7). Data for (A) haematocrit (%), (B) plasma osmolality (mOsm kg–1) and (C) plasma angiotensin concentrations (pmol l–1) are shown (**P<0.01; ***P<0.001, paired t-tests).

View larger version (22K): [in a new window]   Fig. 3. (A) Haematocrit (%), (B) plasma osmolality (mOsm kg–1) and (C) plasma angiotensin concentrations (pmol l–1) of river lampreys acclimated to freshwater at 15 mOsm kg–1. Control lampreys (non-injected; black bars) were held under light anaesthesia for 15 min (N=8) and 30 min (N=4) in the absence of further manipulation. Experimental lampreys were blood sampled at either 15 min or 30 min after an i.p. injection of 1% body mass with either isosmotic saline (white bars; 120 mmol l–1 NaCl; 233 mOsm kg–1; N=5 at each time point) or hyperosmotic saline (cross-hatched bars; 4 mol l–1 NaCl; N=5 at 30 min; N=8 at 30 min). Different letters above error bars signify groups that differ significantly (ANOVA and post-hoc multiple comparison tests).

View larger version (12K): [in a new window]   Fig. 4. Effects of a rapid increase in environmental salinity (FW to 21 p.p.t.; 605 mOsm kg–1) on (A) plasma osmolality (mOsm kg–1), (B) blood haematocrit (%) and (C) plasma angiotensin concentration (pmol l–1) of river lampreys (N=8 at each time point: 0 h, 2 h, 4 h, 8 h and 24 h after transfer). Groups with different letters differ significantly (ANOVA and post-hoc multiple comparison tests). Asterisks signify groups that differ significantly from time 0 h(*P<0.05, **P<0.01, ANOVA followed by linear contrast analyses).

View larger version (12K): [in a new window]   Fig. 5. Effects of a rapid decrease in environmental salinity (from 26 p.p.t., 758 mOsm kg–1 to FW, 22 mOsm kg–1) on river lampreys. Blood samples were collected from a group of lampreys prior to transfer and further groups at 2 h, 4 h, 8 h and 24 h after transfer. Data for (A) plasma osmolality (mOsm kg–1; N=8 at each time point), (B) haematocrit (%; N=10 at 0h, 8 at all other time points) and (C) plasma angiotensin concentration (pmol l–1; N=9 at 0 h, N=8 at 2 h and 24 h, N=7 at 4 h and 8 h) are shown). Groups with different letters above error bars differed significantly (ANOVA and post-hoc multiple comparison tests).

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

Twitter