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First published online August 18, 2005
Journal of Experimental Biology 208, 3341-3347 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01761

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Role of biogenic amines and cHH in the crustacean hyperglycemic stress response

Simonetta Lorenzon, Paolo Edomi, Piero Giulio Giulianini, Romina Mettulio and Enrico Antonio Ferrero^*

BRAIN Center, Department of Biology, University of Trieste, via Giorgieri 7, I-34127 Trieste, Italy

View larger version (12K): [in a new window]   Fig. 1. A standard curve of five standards of 5-HT for ELISA. The x axis represents the log10 concentration value of the five standards. Absorbance was read in a multiwell reader at 450 nm. Correlation coefficient of the fitted line is r=0.9955 (S.D.=0.075, P<0.001).

View larger version (22K): [in a new window]   Fig. 2. Time course (0.5–2 h) of cHH in the eyestalk homogenates, each group pooled from 10 P. elegans, after injection of 5-HT or DA (10–8 mol g–1 live mass) and from the saline-injected control. Values are expressed as means ± S.D. (N=4).

View larger version (14K): [in a new window]   Fig. 3. Time course (0.5–2 h) of cHH in the hemolymph, each group pooled from 10 P. elegans, after injection of 5-HT or DA (10–8 mol g–1 live mass) and from the saline-injected control. Values are expressed as means ± S.D. (N=4).

View larger version (17K): [in a new window]   Fig. 4. Time course (0.5–3 h) of glycemia in the hemolymph of P. elegans after injection of 5-HT or DA (10–8 mol g–1 live mass) and in the saline-injected control group. Values of increment % are expressed as means ± S.D. (N=10).

View larger version (16K): [in a new window]   Fig. 5. Time course (0.5–3 h) of 5-HT in the hemolymph, each group pooled from 10 P. elegans, after exposure to different concentrations of Cu2+ and from the untreated control. Values are expressed as means ± S.D. (N=4).

View larger version (20K): [in a new window]   Fig. 6. Time course (0.5–3 h) of 5-HT in the eyestalk homogenates, each group pooled from 10 P. elegans, after exposure to different concentrations of Cu2+ and from the untreated control. Values are expressed as means ± S.D. (N=4).