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First published online July 6, 2005
Journal of Experimental Biology 208, 2731-2739 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01668
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Renal regulation of plasma glucose in the freshwater rainbow trout

Carol Bucking* and Chris M. Wood

McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada L8S 4K1



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Fig. 1. (A) Plasma glucose concentrations (mmol l–1). (B) Urine glucose concentrations (mmol l–1). Values are means ± 1 S.E.M. (N=7). * indicates a significant difference (P<0.05) between the indicated value and the previous 12 h value; a indicates a significant difference (P<0.05) between the indicated value and the glucose-only infusion value at the same time point.

 


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Fig. 2. (A) Renal clearance ratios of glucose. (B) Renal clearance ratios of Na+. (C) Renal clearance ratios of water. Values are means ± 1 S.E.M. (N=7). * indicates a significant difference (P<0.05) between the indicated value and the 0-12 h value; a indicates a significant difference (P<0.05) between the indicated value and the glucose-only infusion value at the same time point.

 


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Fig. 3. Glucose reabsorption (µmol kg–1 h–1) vs Na+ reabsorption (µmol kg–1 h–1) in fish infused with glucose-only or phlorizin and glucose together. * indicates significantly different (P<0.05) regression line slopes. Points are values for individual fish at different times, not mean values. Regression equations: for glucose only group, y=162.01–[171/(1+0.00004x)227.7] (r2=0.9657) and for glucose + phlorizin treated group, y=0.29x+22.586 (r2=0.2584).

 


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Fig. 4. Glucose filtration (µmol kg–1 h–1) vs glucose reabsorption (µmol kg–1 h–1) in fish infused with glucose-only or phlorizin and glucose together. Points are values for individual fish at different times, not mean values. See text for details. Regression equations: for glucose only group, y=0.200x–35.471 (r2=0.8489) and for glucose + phlorizin treated group, y=0.031x+58.047 (r2=0.018).

 


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Fig. 5. Relationships of glucose filtration, excretion and reabsorption rates to plasma glucose concentration in the rainbow trout, allowing estimation of the in vivo glucose transport maximum from the plateau in glucose reabsorption. Points are for fish infused with glucose only and represent individual fish at different times, not mean values. See text for details. The equation of the regression line for glucose filtered is y=5.547x–2.224 (r2=0.9615, P<0.05), where y is the glucose filtration rate (µmol kg–1 h–1) and x is the plasma glucose concentration (mmol l–1). The equation of the regression line for glucose excreted is y=0.942+log(1.207) x1.207x (r2=0.9321, P<0.01), where y is the glucose excretion rate (µmol kg–1 h–1) and x is the plasma glucose concentration (mmol l–1). The equation of the regression line for glucose reabsorbed is y=–96.182+246.349e–0.5{[(x–33.715)/23.302]2} (r2=0.9149, P<0.05), where y is the glucose reabsorption rate (µmol kg–1 h–1) and x is the plasma glucose concentration (mmol l–1).

 





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