First published online June 16, 2005
Journal of Experimental Biology 208, 2615-2620 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01685
Low temperature acclimated populations of the grain aphid Sitobion avenae retain ability to rapidly cold harden with enhanced fitness
S. J. Powell* and
J. S. Bale
School of Biosciences, University of Birmingham, Edgbaston,
Birmingham B15 2TT, UK

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Fig. 1. Percentage survival (mean ± S.E.M.; N=100) of
first instar and newly moulted pre-reproductive S. avenae reared at
10°C, 72 h after direct exposure to a range of sub-zero temperatures.
Discriminating temperatures for first instar nymphs and newly moulted adult
aphids were determined as 11.5°C and 12.0°C,
respectively. Means with the same letter are not significantly different at
P<0.05 (Tukey multiple comparison test).
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Fig. 2. Percentage survival (mean ± S.E.M.; N=50) of
first instar and newly moulted pre-reproductive S. avenae reared at
10°C, 72 h after rapid cold-hardening (RCH) treatment. Aphids were rapidly
cold-hardened at 0°C for increasing periods of time, prior to exposure to
the discriminating temperature (11.5°C for first instar nymphs and
12°C for newly moulted adults). Means with the same letter are not
significantly different at P<0.05 (Tukey multiple comparison
test).
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Fig. 3. Percentage survival (mean ± S.E.M.; N=50) of
10°C-reared first instar and newly moulted pre-reproductive S.
avenae, 72 h after cooling from 10° to 0°C at various rates,
prior to exposure to the discriminating temperature (11.5°C for
first instar nymphs and 12°C for newly moulted adults). Means with
the same letter are not significantly different at P<0.05 (Tukey
multiple comparison test).
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© The Company of Biologists Ltd 2005