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First published online June 16, 2005
Journal of Experimental Biology 208, 2609-2613 (2005)
Published by The Company of Biologists 2005
doi: 10.1242/jeb.01666
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Antifreeze activity in the gastrointestinal fluids of Arctogadus glacialis (Peters 1874) is dependent on food type

Kim Præbel1,* and Hans Ramløv2

1 University of Tromsø, Norwegian College of Fishery Science, N-9037 Tromsø, Norway
2 Roskilde University, Department of Life Sciences and Chemistry, PO Box 260, DK-4000 Roskilde, Denmark



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  		Fig. 1. The hysteresis freezing points of the gastrointestinal fluids and serum from Arctogadus glacialis that had ingested Boreogadus saida (Bs) and crustaceans (Cr). The dark gray boxes represent the melting point depression due to measured ions, light gray boxes represent the difference between the measured melting point and the melting point depression due to measured ions. The open boxes represent the thermal hysteresis due to AFGP. Data are given as mean ± S.E.M. Statistical significance was tested at the P≤0.05 significance level by using a two-tailed Student's t-test. The food type only had significant influence on the antifreeze activity of the mid-gut fluids.

 


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  		Fig. 2. Native gradient polyacrylamide gel showing the AFGP composition in the gastrointestinal fluids and the serum of Arctogadus glacialis and in the serum of Boreogadus saida. Lane 1, stomach fluid, food: crustaceans; lane 2, mid-gut fluid, food: crustaceans; lane 3, hind-gut fluid, food: crustaceans; lane 4, purified AFGP from Dissostichus mawsoni, used as standard; lane 5, A. glacialis serum; lane 6, B. saida serum; lane 7, stomach fluid, food: B. saida; lane 8, mid-gut fluid, food: B. saida; lane 9, hind-gut fluid, food: B. saida. All lanes were loaded with equal amount of AFGP (250 g). The main AFGP sizes for the D. mawsoni standard are indicated. Arrow a indicates an AFGP synthesized by A. glacialis and that is present in all the gastrointestinal fluids; arrow b shows an AFGP in B. saida serum that can be found in all the gastrointestinal fluids; arrow c indicates that AFGP7 has a higher intensity than the AFGP7 in lane 1 (arrow e); arrow d indicates that AFGP8 has higher intensity than AFGP8 in lane 1 (arrow f); arrow g shows the increase in concentration of the high molecular mass AFGPs in the gastrointestinal tract, which originate in the disappearance of the low molecular mass AFGPs.

 

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© The Company of Biologists Ltd 2005