QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online November 19, 2004
Journal of Experimental Biology 207, 4479-4488 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01316

This Article Summary Full Text Full Text (PDF) An erratum has been published Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pepels, P. P. L. M. Articles by Balm, P. H. M. Search for Related Content PubMed PubMed Citation Articles by Pepels, P. P. L. M. Articles by Balm, P. H. M. Social Bookmarking                         What's this?

Bacterial lipopolysaccharide (LPS) modulates corticotropin-releasing hormone (CRH) content and release in the brain of juvenile and adult tilapia (Oreochromis mossambicus; Teleostei)

P. P. L. M. Pepels^*, S. E. Wendelaar Bonga and P. H. M. Balm

Department of Animal Physiology, Faculty of Sciences, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands

View larger version (17K): [in a new window]   Fig. 1. Depicts the major CRH-ir cells groups and CRH-ir pathways in a sagittal section of the brain of tilapia (original data in Pepels et al., 2002a, 2004). Each circle represents approximately 25 CRH-ir cells. The hypophysiotropic CRH-ir neurons located in the nucleus preopticus (npo), nucleus recessus lateralis (nrl) and nucleus lateralis tuberis (nlt) project into the pituitary (pit). The largest CRH-ir cell group is found in the lateral part of the ventral telencephalon (Vl) and projects mainly into the anterior subdivision of the lateral part of the dorsal telencephalon (Dla). CRH-ir terminals have also been found locally in Vl. Many blood capillaries are found in close proximity of the CRH-ir cells in Vl and a collecting vein is located in the sulcus of Vl (Pepels et al., 2004). In the vagal lobe (LX) CRH-ir terminals are present.

View larger version (28K): [in a new window]   Fig. 2. Effects of injection with saline or E. coli LPS on brain and pituitary tissue CRH levels in tilapia (N=6 in all cases). Open bars indicate pre-confinement values and solid bars indicate values of fish sampled following 24 h confinement. The asterisks (*) indicate confinement effects within a saline- or LPS-treatment group (see Presentation of data and statistics for details). Upper left panel: sagittal overview of the brain of tilapia showing the dissected parts (telencephalon, diencephalon, rhombencephalon and pituitary). The grey areas indicate brain parts that were discarded: tectum and midbrain, and the rostral part of the spinal cord.

View larger version (22K): [in a new window]   Fig. 3. (A) Basal and stimulated in vitro CRH release by telencephalic tissues. Tissues were stimulated for 30 min with 5x10–6 mol l–1 norepinephrine (NE) or 5x10–6 mol l–1 serotonin (5-HT). (B) Maximally stimulated in vitro CRH release by telencephalic tissue stimulated (grey bars) with 5x10–6 mol l–1 norepinephrine (NE) or 56 mmol l–1 K+. Before stimulation, tissues were superfused with control medium or with medium containing LPS (50 µg LPS ml–1).

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

Twitter