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First published online October 21, 2004
Journal of Experimental Biology 207, 4095-4104 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01252

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Presence and properties of cellulase and hemicellulase enzymes of the gecarcinid land crabs Gecarcoidea natalis and Discoplax hirtipes

Stuart M. Linton^* and Peter Greenaway

School of Biological, Earth and Environmental Sciences, The University of NSW, Sydney, NSW 2052, Australia

View larger version (16K): [in a new window]   Fig. 1. Total cellulase activity in digestive juice from G. natalis and D. hirtipes. Activity differed significantly among cellulose concentrations (P<0.05) and between G. natalis and D. hirtipes (two-way ANOVA).

View larger version (20K): [in a new window]   Fig. 2. Activities of endo-ß-1,4-glucanase in digestive juice of G. natalis and D. hirtipes. Enzyme activities were significantly different (P<0.05) between concentrations of carboxymethyl cellulose and between species (two-way ANOVA).

View larger version (19K): [in a new window]   Fig. 3. Activities of ß-1,4-glucosidase activities in digestive juice of G. natalis and D. hirtipes in the presence (stars) and absence (circles) of 20 mmol l-1 glucono-D-lactone. For both G. natalis and D. hirtipes, the ß-1,4-glucosidase activity increased with increasing cellobiose concentrations and was higher in the absence of glucono-D-lactone (P<0.05; two-way ANOVA). For ß-1,4-glucosidase activity from D. hirtipes, there was a significant interaction (P<0.05) between cellobiose concentration and treatment (two-way ANOVA). Uninhibited ß-1,4-glucosidase activities from D. hirtipes were higher than uninhibited ß-glucosidase activities from G. natalis (P<0.05; two-way ANOVA).

View larger version (15K): [in a new window]   Fig. 4. Hanes plots of the activity of total cellulase activity from digestive juice of G. natalis and D. hirtipes. The plot was linear for G. natalis {[Cellulose]/total cellulase activity=8.52x[cellulose]+20.82 (r2=0.624, P=0.001, N=7)} but not for D. hirtipes {[Cellulose]/total cellulase activity=952x[cellulose]–12.659 (r2=0.003, P=0.774, N=10)}.

View larger version (20K): [in a new window]   Fig. 5. Hanes plots of the activity of endo-ß-1,4-glucanase activity from the digestive juice of G. natalis and D. hirtipes. The plot was linear for G. natalis {[CMC]/endo-ß-1,4-glucanase activity= 0.329x[CMC]+2.018 (r2=0.514, P=0.0001, N=7)} but not for D. hirtipes {[CMC]/endo-ß-1,4-glucanase activity=–0.298x[CMC]+ 8.588 (r2=0.041, P=0.2, N=10)}.

View larger version (16K): [in a new window]   Fig. 6. Hanes plots of the activity data for ß-1,4-glucosidase from the digestive juice of G. natalis and D. hirtipes. All plots were linear. G. natalis, uninhibited (circles) {[cellobiose]/ß-1,4-glucosidase activity=1.651x[cellobiose]+9.650 (r2=0.410, P=0.00, N=7)}; G. natalis, inhibited (stars) {[cellobiose]/ß-1,4-glucosidase activity=1.387x[cellobiose]+22.58 (r2=0.387, P=0.002, N=7)}. D. hirtipes, uninhibited (circles) {[cellobiose]/ß-1,4-glucosidase activity=0.480x[cellobiose]+ 2.033 (r2=0.832, P=0.00, N=7)}. D. hirtipes, inhibited (stars) {[cellobiose]/ß-1,4-glucosidase activity=0.464x[cellobiose]+ 10.587 (r2=0.450, P=0.000, N=7)}.