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First published online September 15, 2004
Journal of Experimental Biology 207, 3775-3784 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01211
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Time-dependent expression of heat shock proteins 70 and 90 in tissues of the anoxic western painted turtle

Valeria Ramaglia and Leslie T. Buck*

Department of Zoology, University of Toronto, Toronto, ON, Canada, M5S 3G5



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  		Fig. 1. Blood lactate and glucose levels in control, dived and recovery turtles. Blood lactate (circles) and glucose (squares) concentrations in control turtles and turtles force-dived for 2, 6, 12, 18, 24 and 30 h at 17°C. Values are means ± S.E.M. of 5 (control) and 4 (dive) replicates. *Statistical significance (P<0.05) from control animals. Note that symbols obscure error bars in some instances. {dagger}Statistical significance between 24 h and 30 h dived animals (P<0.05).

 


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  		Fig. 2. Expression of Hsp73 following a forced dive. Expression of Hsp73 in tissues from turtles force-dived for 2, 6, 12, 18, 24 and 30 h. Values are means ± S.E.M. of 5 (control) and 4 (dive) replicates. *Statistical significance (P<0.05) from control animals. Note that symbols obscure error bars in some instances.

 


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  		Fig. 3. Expression of Hsp73 following recovery from a forced dive. (A) Representative western blots of Hsp73 expression in turtle brain (B), heart (H), liver (L) and muscle (M) in normoxia (control), after a 30 h forced dive and following 1 h of recovery. (B) Quantitative analysis of Hsp73 expression in tissues from control turtles, turtles force-dived for 12, 24 and 30 h and turtles recovered for 1 h following a 12, 24 and 30 h dive. Values are means ± S.E.M. of 5 (control) and 4 (dive; recovery) replicates. *Statistical significance (P<0.05) from control animals; {dagger}statistical significance between dived animals and the respective recovery animals (P<0.05). Note that the error bars are obscured in some instances.

 


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  		Fig. 4. Expression of Hsp72 following a forced dive. Expression of Hsp72 in tissues from turtles force-dived for 2, 6, 12, 18, 24 and 30 h. Values are means ± S.E.M. of 5 (control) and 4 (dive) replicates. *Statistical significance (P<0.05) from control animals. Note that error bars are obscured in some instances.

 


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  		Fig. 5. Expression of Hsp72 following recovery from a forced dive. (A) Representative western blots of Hsp72 expression in turtle brain (B), heart (H), liver (L) and muscle (M) in normoxia (control), after a 30 h forced dive and following 1 h of recovery. (B) Quantitative analysis of Hsp72 expression in tissues from control turtles, turtles force-dived for 12, 24 and 30 h and turtles recovered for 1 h following a 12, 24 and 30 h dive. Values are means ± S.E.M. of 5 (control) and 4 (dive; recovery) replicates. *Statistical significance (P<0.05) from control animals; {dagger}statistical significance between dived animals and the respective recovery animals (P<0.05).

 


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  		Fig. 6. Expression of Hsp90 following a forced dive. Expression of Hsp90 in tissues from turtles force-dived for 2, 6, 12, 18, 24 and 30 h. Values are means ± S.E.M. of 5 (control) and 4 (dive) replicates. *Statistical significance (P<0.05) from control animals. Note that symbols obscure error bars in some instances.

 


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  		Fig. 7. Expression of Hsp90 following recovery from a forced dive. (A) Representative western blots of Hsp90 expression in turtle brain (B), heart (H), liver (L) and muscle (M) in normoxia (control), after 30 h dive and following 1 h of recovery. (B) Quantitative analysis of Hsp90 expression in tissues from control turtles, turtles force-dived for 12, 24 and 30 h and turtles recovered for 1 h following a 12, 24 and 30 h dive. Values are means ± S.E.M. of 5 (control) and 4 (dive; recovery) replicates. *Statistical significance (P<0.05) from control animals; {dagger}statistical significance between dived animals and the respective recovery animals (P<0.05). Note that error bars are obscured in some instances.

 

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© The Company of Biologists Ltd 2004