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First published online December 10, 2003
Journal of Experimental Biology 207, 357-367 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.00747

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Seasonality of the red blood cell stress response in rainbow trout (Oncorhynchus mykiss)

P. Koldkjær^1,*, T. G. Pottinger², S. F. Perry³ and A. R. Cossins¹

¹ School of Biological Sciences, University of Liverpool, The Biosciences Building, Crown Street, Liverpool L69 7ZB, UK
² CEH Windermere, The Ferry House, Far Sawrey, Ambleside, Cumbria LA22 0LP, UK
³ Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5

View larger version (27K): [in a new window]   Fig. 2. Seasonality in the ß-adrenergically stimulated red blood cell (RBC) Na+ influx (A), daily ambient water temperature (B) and plasma cortisol levels (C). The dotted line in A illustrates the mean Na influx at the times of the year when the ß-NHE activity is not downregulated. The crosshatched boxes on the x-axis indicate the spawning periods, defined by high proportions of ovulated females. Values are means ± S.E.M. (N=8).

View larger version (33K): [in a new window]   Fig. 1. Seasonal variation in the number of ß-adrenergic receptors (ß-ARs; grey bars) and daily ambient water temperature (black line) throughout the study. Values are means ± S.E.M. (N=8). The inset shows the linear regression curve for ß-AR number vs mean water temperature (P=0.002, f(x)=474.7+74.1x, r2=0.417). Water temperature in the inset was calculated from the temperatures measured over the full period since the previous sampling.

View larger version (22K): [in a new window]   Fig. 3. The seasonal variations in ß-adrenergic receptor (ß-AR) numbers (filled circles) and ß3b-AR mRNA levels (relative to the August value; open circles). Values are means ± S.E.M. (N=8 for ß-AR numbers and N=3-8 for ß3b-AR mRNA values). The inset illustrates the linear regression curve for receptor number vs ß3b-AR mRNA levels (P=0.005, f(x)=734.5+1252.8x, r2=0.755).

View larger version (22K): [in a new window]   Fig. 4. Seasonal changes in the ß-adrenergically stimulated RBC Na+/H+ exchanger (ßNHE) activity (filled circles) and ßNHE mRNA levels (relative to August value; open circles). Note one outlying ßNHE mRNA value is plotted separately. Values are means ± S.E.M. (N=8 for ßNHE activity values and N=3-8 for ßNHE mRNA values). The inset shows the linear regression curve for ßNHE activity vs ßNHE mRNA values (P=0.038, f(x)=135.0x/(0.274+x), r2=0.538).

View larger version (27K): [in a new window]   Fig. 5. Seasonal fluctuations in plasma testosterone (A) and plasma oestradiol-17ß concentrations (C) in relation to fluctuations in red blood cell (RBC) ß-adrenergically stimulated RBC Na+/H+ exchanger (ßNHE) activity (B). The dotted line illustrates the mean Na+ influx at the times of the year when the ß-NHE activity is not downregulated. Values are means ± S.E.M. (N=8).

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