QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online August 23, 2004
Journal of Experimental Biology 207, 3419-3430 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01166

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Van Anholt, R. D. Articles by Bonga, S. E. W. Search for Related Content PubMed PubMed Citation Articles by Van Anholt, R. D. Articles by Bonga, S. E. W.

Arachidonic acid reduces the stress response of gilthead seabream Sparus aurata L.

R. D. Van Anholt^1,*, F. A. T. Spanings¹, W. M. Koven², O. Nixon² and S. E. Wendelaar Bonga¹

¹ Department of Animal Ecology and Ecophysiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands
² Department of Larval Rearing, The National Center for Mariculture, PO Box 1212, Eilat 88112, Israel

View larger version (12K): [in a new window]   Fig. 1. The effect of a diet low in arachidonic acid (low-ArA) and an ArA-enriched diet (high-ArA) in combination with acetylsalicylic acid (ASA) treatment on plasma cortisol responses to 5 min net confinement. Open circles, low-ArA; filled circles, low-ArA + ASA; open triangles, high-ArA; filled triangles, high-ArA + ASA. Samples were taken from non-stressed fish (t=0) and at several intervals after confinement (t=20 min, 60 min, 24 h). ASA-treated fish were subjected to confinement 4 h after the last dose of ASA. Each time point represents the mean value ± S.E.M. of 10 fish. The results of both graphs were combined for analysis and significant effects of the factors: ArA supplementation, ASA treatment, time after confinement, and/or interactions are indicated: *P<0.05, **P<0.01, ***P<0.001 (3-way ANOVA).

View larger version (12K): [in a new window]   Fig. 2. The effect of ArA, ASA and net confinement on plasma glucose levels. Samples were taken from non-stressed fish (t=0) and at intervals after confinement (t=20 min, 60 min, 24 h). Open circles, low-ArA; filled circles, low-ArA + ASA; open triangles, high-ArA; filled triangles, high-ArA + ASA. ASA-treated fish were subjected to confinement 4 h after the last dose of ASA. Values are means ± S.E.M., N=10.

View larger version (12K): [in a new window]   Fig. 3. The effect of ArA, ASA and net confinement on plasma lactate levels. Samples were taken from non-stressed fish (t=0) and at intervals after confinement (t=20 min, 60 min, and 24 h). Open circles, low-ArA; filled circles, low-ArA + ASA; open triangles, high-ArA; filled triangles, high-ArA + ASA. ASA-treated fish were subjected to confinement 4 h after the last dose of ASA. Values are means ± S.E.M., N=10.

View larger version (14K): [in a new window]   Fig. 4. Changes in plasma osmolality after feeding different diets and ASA administration. Samples were taken just prior to (t=0) and at intervals after net confinement (t=20 min, 60 min and 24 h). Open circles, low-ArA; filled circles, low-ArA + ASA; open triangles, high-ArA; filled triangles, high-ArA + ASA. ASA treated fish were subjected to confinement 4 h after the last dose of ASA. Values are means ± S.E.M., N=10.

View larger version (22K): [in a new window]   Fig. 5. Changes in plasma ions levels after feeding different diets and ASA administration. (A) Sodium, (B) chloride, (C) potassium. Samples were taken from non-stressed fish (t=0) and after net confinement (for 20 min, 60 min and 24 h): Open circles, low-ArA; filled circles, low-ArA + ASA; open triangles, high-ArA; filled triangles, high-ArA + ASA. Values are means ± S.E.M., N=10.

View larger version (18K): [in a new window]   Fig. 6. Basal gill Na+, K+-ATPase activity (µmol Pi h-1 mg-1 protein) before (t=0 h) and 24 h after net confinement in control and ArA- and ASA-fed seabream. Samples at t=0 were collected 4 h after the last dose of ASA in the ASA-treated fish. Values are means ± S.E.M., N=10.

View larger version (13K): [in a new window]   Fig. 7. The inhibition of Na+, K+-ATPase activity by free ArA in the incubation medium. Values are means ± S.E.M., N=10. Na+, K+-ATPase activity in 1% methanol was set at 100%. Activities were compared for significance to the activity at 1% methanol and 0 µmol l-1 ArA (see Materials and methods for details); *P<0.05, **P<0.01, ***P<0.001.